Marine Biotechnology

, Volume 10, Issue 6, pp 664–675 | Cite as

Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio

  • Bing Wang
  • Fuhua Li
  • Wei Luan
  • Yusu Xie
  • Chengsong Zhang
  • Zhan Luo
  • Lang Gui
  • Hui Yan
  • Jianhai Xiang
Original Article

Abstract

Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.

Keywords

WSSV Vibrio cDNA microarray Fenneropenaeus chinensis 

Notes

Acknowledgements

This work is financially supported by the Major State Basic Research Development Program of China (973 program) 2006CB101804, National Natural Science Foundation of China (30771639), and National 863 High-tech Program of China (2007AA09Z430, 2006AA10A402).

References

  1. Adachi K, Wakamatsu K, Ito S, Miyamoto N, Kokubo T, Nishioka T, Hirata T (2006) An oxygen transporter hemocyanin can act on the late pathway of melanin synthesis. Pigment Cell Res 19:214–219CrossRefGoogle Scholar
  2. Adams JC (2001) Thrombospondins: multifunctional regulators of cell interactions. Annu Rev Cell Dev Biol 17:25–51PubMedCrossRefGoogle Scholar
  3. Arts JAJ, Cornelissen FHJ, Cijsouw T, Hermsen T, Savelkoul HFJ, Stet RJM (2006) Molecular cloning and expression of a Toll receptor in the giant tiger shrimp, Penaeus monodon. Fish Shellfish Immunol 23:504–513PubMedCrossRefGoogle Scholar
  4. Auslander M, Yudkovski Y, Chalifa-Caspi V, Herut B, Ophir R, Reinhardt R, Neumann PM, Tom M (2008) Pollution-affected fish hepatic transcriptome and its expression patterns on exposure to cadmium. Mar Biotechnol (NY) 10:250–261CrossRefGoogle Scholar
  5. Bachere E (2000) Shrimp immunity and disease control. Aquaculture 191:3–11CrossRefGoogle Scholar
  6. Bachere E, Chagot D, Grizel H (1988) Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation. Dev Comp Immunol 12:549–559PubMedCrossRefGoogle Scholar
  7. Bachere E, Gueguen Y, Gonzalez M, de Lorgeril LJ, Garnier J, Romestand B (2004) Insights into the anti-microbial defense of marine invertebrates: the penaeid shrimps and the oyster Crassostrea gigas. Immunol Rev 198:149–168PubMedCrossRefGoogle Scholar
  8. Baticados MCL, Lavilla PCR, Cruz LER, Dela PLD, Sunaz NA (1990) Studies on the chemical control of luminous bacteria Vibrio harveyi and V. splendidus isolated from diseased Penaeus monodon larvae and rearing water. Dis Aquat Org 9:133–139CrossRefGoogle Scholar
  9. Bechteler C, Holler D (1995) [Preliminary studies of the immunization of shrimp (Penaeus monodon) against Vibrio infections]. Berl Munch Tierarztl Wochenschr 108:462–465PubMedGoogle Scholar
  10. Bright SI, Manjusha M, Pai SS, Philip R (2005) Fenneropenaeus indicus is protected from white spot disease by oral administration of inactivated white spot syndrome virus. Dis Aquat Organ 66:265–270CrossRefGoogle Scholar
  11. Cheng W, Liu CH, Tsai CH, Chen JC (2005) Molecular cloning and characterisation of a pattern recognition molecule, lipopolysaccharide- and beta-1,3-glucan binding protein (LGBP) from the white shrimp Litopenaeus vannamei. Fish Shellfish Immunol 18:297–310PubMedCrossRefGoogle Scholar
  12. Cheshenko N, Del Rosario B, Woda C, Marcellino D, Satlin LM, Herold BC (2003) Herpes simplex virus triggers activation of calcium-signaling pathways. J Cell Biol 163:283–293PubMedCrossRefGoogle Scholar
  13. Clynen E, Schoofs L, Salzet M (2005) A review of the most important classes of serine protease inhibitors in insects and leeches. Med Chem Rev 2:1–10Google Scholar
  14. Cohen R, Chalifa-Caspi V, Williams TD, Auslander M, George SG, Chipman JK, Tom M (2008) Estimating the efficiency of fish cross-species cDNA microarray hybridization. Mar Biotechnol (NY) 9:491–499CrossRefGoogle Scholar
  15. Darias MJ, Zambonino-Infante JL, Hugot K, Cahu CL, Mazurais D (2008) Gene expression patterns during the larval development of European sea bass (Dicentrarchus labrax) by microarray analysis. Mar Biotechnol (NY) (in press)Google Scholar
  16. Destoumieux D, Munoz M, Bulet P, Bachere E (2000) Penaeidins, a family of antimicrobial peptides from penaeid shrimp (Crustacea, Decapoda). Cell Mol Life Sci 57:1260–1271PubMedCrossRefGoogle Scholar
  17. Du XJ, Wang JX, Liu N, Zhao XF, Li FH, Xiang JH (2006) Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis). Mol Immunol 43:1633–1644PubMedCrossRefGoogle Scholar
  18. Duda DM, Tu C, Fisher SZ, An H, Yoshioka C, Govindasamy L, Laipis PJ, Agbandje-McKenna M, Silverman DN, Mckenna R (2005) Human carbonic anhydrase III: structural and kinetic study of catalysis and proton transfer. Biochemistry 44:10046–10053PubMedCrossRefGoogle Scholar
  19. George MR, Maharajan A, John KR, Prince JMJ (2006) Shrimps survive white spot syndrome virus challenge following treatment with Vibrio bacterin. Indian J Exp Biol 44:63–67PubMedGoogle Scholar
  20. Gordon JC, Yuehan Z, Patrice B, Irina IG, Walter FB (2002) Transport of volatile solutes through AQP1. J Physiol 542(Pt 1):17–29Google Scholar
  21. Hasenstein JR, Zhang GL, Lamont SJ (2006) Analyses of five Gallinacin genes and the Salmonella enterica serovar Enteritidis response in poultry. Infect Immun 7:3375–3380CrossRefGoogle Scholar
  22. Ishii Y, Akazawa D, Aoki Y, Yamada H, Oguri H (2005) Suppression of carbonic anhydrase III mRNA level by an aryl hydrocarbon receptor ligand in primary cultured hepatocytes of rat. Biol Pharm Bull 28:1087–1090PubMedCrossRefGoogle Scholar
  23. Janeway CA, Travers P, Walport M, Shlomchik M (2001) Immunobiology: the immune system in health and disease. Garland Science, New YorkGoogle Scholar
  24. Jarasrassamee B, Supungul P, Panyim S, Klinbunga S, Rimphanichayakit V, Tassanakajon A (2005) Recombinant expression and characterization of five-domain Kazal-type serine proteinase inhibitor of black tiger shrimp (Penaeus monodon). Mar Biotechnol (NY) 7:46–52CrossRefGoogle Scholar
  25. Jenny MJ, Chapman RW, Mancia A, Chen YA, McKillen DJ, Trent H, Lang P, Escoubas JM, Bachere E, Boulo V, Liu ZJ, Gross PS, Cunningham C, Cupit PM, Tanguy A, Guo X, Moraga D, Boutet I, Huvet A, De Guise S, Almeida JS, Warr GW (2007) A cDNA microarray for Crassostrea virginica and C. gigas. Mar Biotechnol (NY) 9:577–591CrossRefGoogle Scholar
  26. Johansson KC, Metzendorf C, Soderholl K (2005) Microarray analysis of immune challenged Drosophila hemocytes. Exp Cell Res 305:145–155PubMedCrossRefGoogle Scholar
  27. Kochzius M, Nolte M, Weber H, Silkenbeumer N, Hjorleifsdottir S, Hreggvidsson GO, Marteinsson V, Kappel K, Planes S, Tinti F, Magoulas A, Garcia Vazquez E, Turan C, Hervet C, Campo Falgueras D, Antoniou A, Landi M, Blohm D (2008) DNA microarrays for identifying fishes. Mar Biotechnol (NY) 10:207–217CrossRefGoogle Scholar
  28. Laurent BC, Treitel MA, Carmean D (1990) The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes. Mol Cell Biol 10:56–5625Google Scholar
  29. Lee SY, Soderhall K (2006) Early events in crustacean innate immunity. Fish Shellfish Immunol 12:421–437Google Scholar
  30. Lehming N, Thanos D, Brickman JM, Ma J, Maniatis T, Ptashne M (1994) An HMG-like protein that can switch a transcriptional activator to a repressor. Nature 371:175–179PubMedCrossRefGoogle Scholar
  31. Lightner DV (1996) Epizootiology, distribution and the impact on international trade of two penaeid shrimp viruses in the Americas. Rev Sci Tech 15:579–601PubMedGoogle Scholar
  32. Liu CH, Tseng DY, Lai CY, Cheng W, Kuo CM (2006) Molecular cloning and characterisation of prophenoloxidase cDNA from haemocytes of the giant freshwater prawn, Macrobrachium rosenbergii, and its transcription in relation with the moult stage. Fish Shellfish Immunol 21:60–69PubMedCrossRefGoogle Scholar
  33. Liu F, Liu Y, Li F, Dong B, Xiang J (2005) Molecular cloning and expression profile of putative antilipopolysaccharide factor in Chinese shrimp(Fenneropenaeus chinensis). Mar Biotechnol (NY) 7:600–608CrossRefGoogle Scholar
  34. Liu YC, Li FH, Dong B, Wang B, Luan W, Zhang XJ, Zhang LS, Xiang JH (2007) Molecular cloning, characterization and expression analysis of a putative C-type lectin (Fclectin) gene in Chinese shrimp Fenneropenaeus chinensis. Mol Immunol 44:598–607PubMedCrossRefGoogle Scholar
  35. Lu KY, Huang YT, Lee HH, Sung HH (2006) Cloning the prophenoloxidase cDNA and monitoring the expression of proPO mRNA in prawns (Macrobrachium rosenbergii) stimulated in vivo by CpG oligodeoxynucleotides. Fish Shellfish Immunol 20:274–284PubMedCrossRefGoogle Scholar
  36. Luan W, Li FH, Zhang JQ, Wang B, Xiang JH (2007) Cloning and expression of glucose regulated protein 78 (GRP78) in Fenneropenaeus chinensis. Mol Biol Rep (in press)Google Scholar
  37. Moriya S, Sato S, Azumaya T, Suzuki O, Urawa S, Urano A, Abe S (2007) Genetic stock identification of chum salmon in the Bering Sea and North Pacific Ocean using mitochondrial DNA microarray. Mar Biotechnol (NY) 9:179–191CrossRefGoogle Scholar
  38. Moyer SA, Baker SC, Lessard JL (1986) Tubulin: a factor necessary for the synthesis of both Sendai virus and vesicular stomatitis virus RNA. Proc Natl Acad Sci U S A 83:5405–5409PubMedCrossRefGoogle Scholar
  39. Moyer SA, Baker SC, Horikami SM (1990) Host cell proteins required for measles virus reproduction. J Gen Virol 71:775–83PubMedCrossRefGoogle Scholar
  40. Nishida E, Kumagai H (1980) Calcium sensitivity of sea urchin tubulin in in vitro assembly and the effects of calcium-dependent regulator (CDR) proteins isolated from sea urchin eggs and porcine brains. J Biochem 87:143–151PubMedGoogle Scholar
  41. Oleksiak MF, Kolell KJ, Crawford DL (2001) Utility of natural populations for microarray analyses: isolation of genes necessary for functional genomic studies. Mar Biotechnol (NY) 3:S203–211CrossRefGoogle Scholar
  42. Orlacchio A, Martino S, Sarchielli P, Gallai A, Emiliani C (1998) Beta-N-acetylhexosaminidase in peripheral blood lymphocytes and monocytes in the different forms and stages of multiple sclerosis. J Neurochem 71:1168–1176PubMedCrossRefGoogle Scholar
  43. Robalino J, Payne C, Parnell P, Shepard E, Grimes AC, Metz A, Prior S, Witteveldt J, Vlak JM, Gross PS, Warr G, Browdy CL (2006) Inactivation of white spot syndrome virus (WSSV) by normal rabbit serum: implications for the role of the envelope protein VP28 in WSSV infection of shrimp. Virus Res 118:55–61PubMedCrossRefGoogle Scholar
  44. Roy G, De Crescenzo G, Khaleghpour K, Kahvejian A, O’Connor-McCourt M, Sonenshine DE (2002) Paip1 interacts with poly(A) binding protein through two independent binding motifs. Mol Cell Biol 22:3769–3782PubMedCrossRefGoogle Scholar
  45. Schena M, Shalon D, Davis RW, Brown PO (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270:467–470PubMedCrossRefGoogle Scholar
  46. Somboonwiwat K, Supungul P, Rimphanitchayakit V, Aoki T, Hirono I, Tassanakajon A (2006) Differentially expressed genes in hemocytes of Vibrio harveyi-challenged shrimp Penaeus monodon. J Biochem Mol Biol 39:26–36PubMedGoogle Scholar
  47. Tassi C, Mancuso F, Gambelunghe C, Rufini S, Feligioni L, Biondi R, Rossi R, Capodicasa E (2001) Beta-N-acetylhexosaminidase activity and isoenzyme profile in the kidney and urine of trained rats. Immunopharmacol Immunotoxicol 23:573–583PubMedCrossRefGoogle Scholar
  48. Tsoi SC, Cale JM, Bird IM, Ewart V, Brown LL, Douglas S (2003) Use of human cDNA microarrays for identification of differentially expressed genes in Atlantic salmon liver during Aeromonas salmonicida infection. Mar Biotechnol (NY) 5:545–554CrossRefGoogle Scholar
  49. Vaseeharan B, Prem AT, Murugan T, Chen JC (2006) Shrimp vaccination trials with the VP292 protein of white spot syndrome virus. Lett Appl Microbiol 43:137–142PubMedCrossRefGoogle Scholar
  50. Wang B, Li F, Dong B, Zhang X, Zhang C, Xiang J (2006a) Discovery of the genes in response to white spot syndrome virus (WSSV) infection in Fenneropenaeus chinensis through cDNA microarray. Mar Biotechnol (NY) 8:491–500CrossRefGoogle Scholar
  51. Wang B, Li FH, Xie YS, Xiang JH (2006b) The full length cDNA cloning and expression profile of thrombospondin of Fenneropenaeus chinensis (in chiese). High Technology Letters 16:78–83Google Scholar
  52. Witteveldt J, Vlak JM, van Hulten MC (2006) Increased tolerance of Litopenaeus vannamei to white spot syndrome virus (WSSV) infection after oral application of the viral envelope protein VP28. Dis Aquat Org 70:167–170PubMedCrossRefGoogle Scholar
  53. Wynne JW, O’Sullivan MG, Cook MT, Stone G, Nowak BF, Lovell DR, Elliott NG (2008) Transcriptome analyses of amoebic gill disease-affected Atlantic salmon (Salmo salar) tissues reveal localized host gene suppression. Mar Biotechnol (NY) (in press)Google Scholar
  54. Yang F, He J, Lin X, Li Q, Pan D, Zhang X, Xu X (2001) Complete genome sequence of the shrimp white spot bacilliform virus. J Virol 75:11811–11820PubMedCrossRefGoogle Scholar
  55. Zhang QL, Li HL, Wang B, Zhang JQ, Liu YC, Zhou Q, Xiang JH (2007) The mitochondrial manganese superoxide dismutase gene in Chinese shrimp Fenneropenaeus chinensis: cloning, distribution and expression. Dev Comp Immunol 31:429–440PubMedCrossRefGoogle Scholar
  56. Zimmerman UJ, Wang P, Zhang X, Bogdanovich S, Forster R (2004) Anti-oxidative response of carbonic anhydrase III in skeletal muscle. IUBMB Life 56:343–347PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Bing Wang
    • 1
  • Fuhua Li
    • 1
  • Wei Luan
    • 1
    • 2
  • Yusu Xie
    • 1
    • 2
  • Chengsong Zhang
    • 1
    • 2
  • Zhan Luo
    • 1
    • 2
  • Lang Gui
    • 1
    • 2
  • Hui Yan
    • 1
    • 2
  • Jianhai Xiang
    • 1
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  2. 2.Graduate SchoolChinese Academy of SciencesBeijingPeople’s Republic of China

Personalised recommendations