Marine Biotechnology

, Volume 10, Issue 4, pp 438–446 | Cite as

The Encysted Dormant Embryo Proteome of Artemia sinica

  • Qian Zhou
  • Changgong Wu
  • Bo Dong
  • Fengqi Liu
  • Jianhai Xiang
Original Article
First Online:
Received:
Revised:
Accepted:

Abstract

The possibility of the brine shrimp Artemia to produce dormant embryo (cysts) in diapause is a key feature in its life history. In the present study, we obtained a proteomic reference map for the diapause embryo of Artemia sinica using two-dimensional gel electrophoresis with a pH range of 4–7 and a molecular weight range of 10–100 kDa. Approximately 233 proteins were detected, and 60 of them were analyzed by capillary liquid chromatography tandem mass spectrometry (LC–MS/MS). Of these, 39 spots representing 33 unique proteins were identified, which are categorized into functional groups, including cell defense, cell structure, metabolism, protein synthesis, proteolysis, and other processes. This reference map will contribute toward understanding the state of the diapause embryo and lay the basis and serve as a useful tool for further profound studies in the proteomics of Artemia at different developmental stages and physiological conditions.

Keywords

Proteome Diapause embryo 2D-E Mass spectrometry Artemia sinica 
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Notes

Acknowledgments

This research was supported by the program of National Natural Science Foundation of China (20060109Z4016) and Natural Science Foundation of Shandong Province for the excellent young researcher (2006BSA02004).

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Qian Zhou
    • 1
    • 2
  • Changgong Wu
    • 1
  • Bo Dong
    • 1
  • Fengqi Liu
    • 3
  • Jianhai Xiang
    • 1
  1. 1.Key Laboratory of Experimental Marine Biology, Institute of OceanologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  2. 2.Graduate SchoolChinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.College of Life SciencesNankai UniversityTianjinPeople’s Republic of China

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