Gene Expression Patterns During the Larval Development of European Sea Bass (Dicentrarchus Labrax) by Microarray Analysis
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During the larval period, marine teleosts undergo very fast growth and dramatic changes in morphology, metabolism, and behavior to accomplish their metamorphosis into juvenile fish. Regulation of gene expression is widely thought to be a key mechanism underlying the management of the biological processes required for harmonious development over this phase of life. To provide an overall analysis of gene expression in the whole body during sea bass larval development, we monitored the expression of 6,626 distinct genes at 10 different points in time between 7 and 43 days post-hatching (dph) by using heterologous hybridization of a rainbow trout cDNA microarray. The differentially expressed genes (n = 485) could be grouped into two categories: genes that were generally up-expressed early, between 7 and 23 dph, and genes up-expressed between 25 and 43 dph. Interestingly, among the genes regulated during the larval period, those related to organogenesis, energy pathways, biosynthesis, and digestion were over-represented compared with total set of analyzed genes. We discuss the quantitative regulation of whole-body contents of these specific transcripts with regard to the ontogenesis and maturation of essential functions that take place over larval development. Our study is the first utilization of a transcriptomic approach in sea bass and reveals dynamic changes in gene expression patterns in relation to marine finfish larval development.
KeywordsFish larvae Development Microarray Gene expression Sea bass
The authors thank INRA-GADIE resource center for providing microarrays (Jouy en Josas, France) and Genofish program for financial and scientific support. We also thank A. Le Cam and J. Montfort from INRA-SCRIBE genomic platform (Rennes, France) for excellent technical assistance, SIGENAE team (INRA Toulouse, France) for bioinformatic tools development, P. Quazuguel, and Gabriela Hortopan for larval rearing and real-time PCR analysis, respectively, and Helen McCombie for correcting the English. M. J. Darias was supported by a postdoctoral fellowship from the Fundación Ramón Areces (Spain).
- Horiuchi K, Amizuka N, Takeshita S, Takamatsu H, Katsuura M, Ozawa H, Toyama Y, Bonewald LF, Kudo A (1999) Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. J Bone Miner Res 14:1239–1249PubMedCrossRefGoogle Scholar
- Jenny MJ, Chapman RW, Mancia A, Chen YA, McKillen DJ, Trent H, Lang P, Escoubas JM, Bachere E, Boulo V, Liu ZJ, Gross PS, Cunningham C, Cupit PM, Tanguy A, Guo X, Moraga D, Boutet I, Huvet A, De Guise S, Almeida JS, Warr GW (2007) A cDNA microarray for Crassostrea virginica and C. gigas. Mar Biotechnol 9:577–591PubMedCrossRefGoogle Scholar
- Meyer RA, Sweeney HL, Kushmerick MJ (1984) A simple analysis of the “phosphocreatine shuttle”. Am J Physiol 246:365–377Google Scholar
- Mori T, Hiraka I, Kurata Y, Kawachi H, Mano N, Devlin RH, Nagoya H, Araki K (2007) Changes in hepatic gene expression related to innate immunity, growth and iron metabolism in GH-transgenic amago salmon (Oncorhynchus masou) by cDNA subtraction and microarray analysis, and serum lysozyme activity. Gen Comp Endocrinol 151:42–54PubMedCrossRefGoogle Scholar
- Moriya S, Sato S, Azumaya T, Suzuki O, Urawa S, Urano A, Abe S (2007) Genetic stock identification of chum salmon in the Bering Sea and North Pacific Ocean using mitochondrial DNA microarray. Mar Biotechnol 9:179–191Google Scholar
- Person-LeRuyet J, Fischer C, Mugnier C (1991) Potentiel de croissance du bar (Dicentrarchus labrax) pendant la phase ecloserie: relation tailles/poids. CIEM F 38:16–31Google Scholar
- Tanaka TS, Jaradat SA, Lim MK, Kargul GJ, Wang X, Grahovac MJ, Pantano S, Sano Y, Piao Y, Nagaraja R, Doi H, Wood WH 3rd, Becker KG, Ko MS (2000) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray. Proc Natl Acad Sci U S A 97:9127–9132PubMedCrossRefGoogle Scholar
- Villeneuve LA, Gisbert E, Moriceau J, Cahu CL, Zambonino-Infante JL (2006) Intake of high levels of vitamin A and polyunsaturated fatty acids during different developmental periods modifies the expression of morphogenesis genes in European sea bass (Dicentrarchus labrax). Br J Nutr 95:677–687PubMedCrossRefGoogle Scholar