Marine Biotechnology

, Volume 10, Issue 3, pp 242–249 | Cite as

Oral Administration of Bacterially Expressed VP28dsRNA to Protect Penaeus monodon from White Spot Syndrome Virus

  • M. Sarathi
  • Martin C. Simon
  • C. Venkatesan
  • A. S. Sahul HameedEmail author
Original Article


We explored the possibility of protecting Penaeus monodon against white spot syndrome virus (WSSV) infection via interference RNA technology by oral administration of bacterially expressed WSSV VP28dsRNA. Shrimp were given dsRNA orally via two methods. In the first method, pellet feed was coated with inactivated bacteria containing overexpressed dsRNA of the WSSV VP28 gene, and in the second method, pellet feed was coated with VP28dsRNA–chitosan complex nanoparticles. The treated shrimp were orally challenged with WSSV by feeding WSSV-infected tissue. The experiment was conducted for 30 days. The dsRNA-treated shrimp challenged with WSSV showed higher survival compared to control shrimp. Sixty-eight percent survival was observed in shrimp fed with feed coated with inactivated bacteria containing dsRNA of the WSSV VP28 gene whereas 37% survival was observed in shrimp fed with VP28dsRNA–chitosan complex nanoparticle–coated feed. The WSSV caused 100% mortality in shrimp fed with pellet feed coated with inactivated bacteria with empty LITMUS38i vector. At the end of the experiment, the tissue samples prepared from randomly selected shrimp that survived were analyzed via reverse transcriptase-polymerase chain reaction and Western blot analysis for WSSV. The samples were negative for WSSV. Based on the present data and the advantages of dsRNA, we believe that oral administration of crude extract of bacterially expressed VP28dsRNA is a potential therapeutic agent against WSSV infection of shrimp.


Antiviral defense Chitosan Oral administration RNAi VP28dsRNA White spot syndrome virus 



M. Sarathi is a recipient of a Senior Research Fellowship award from the Council of Scientific and Industrial Research (CSIR), New Delhi. This study was funded by the Department of Biotechnology, Government of India, New Delhi. The authors thank the Management of C. Abdul Hakeem College, Melvisharam for providing the facilities to carry out this work.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • M. Sarathi
    • 1
  • Martin C. Simon
    • 2
  • C. Venkatesan
    • 1
  • A. S. Sahul Hameed
    • 1
    Email author
  1. 1.Aquaculture Biotechnology Division, Department of ZoologyC. Abdul Hakeem CollegeMelvisharamIndia
  2. 2.Department of BiologyUniversity of KaiserslauternKaiserslauternGermany

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