Isolation and Characterization of Two Groups of Novel Marine Bacteria Producing Violacein
Thirteen strains of novel marine bacteria producing a purple pigment were isolated from the Pacific coast of Japan. They were divided into two groups based on their 16S ribosomal RNA gene sequences, and both groups of bacteria belonged to the genus Pseudoalteromonas. The UV-visible spectrum of the pigment was identical to those of violacein, a pigment produced by several species of bacteria including Chromobacterium violaceum, an opportunistic pathogen. Further analysis of the chemical structure of the pigment by mass spectroscopy and nuclear magnetic resonance spectroscopy showed that the pigment was violacein. The high purity of violacein in the crude extract enabled us to employ simple and nonpolluting procedures to purify the pigment. Isolated bacteria may be useful as a C. violaceum substitute for the safe production of violacein.
Keywordspigment Pseudoalteromonas violacein
We thank Professors Masaoki Furue, Kazuya Kobiro and Takahiro Hosokawa of the Kochi University of Technology for their valuable discussions and encouragement. We also thank Ms. Kimi Minami, Mr. Zhao Yong Bian, and Mr. Tomoki Shirai for their assistance during NMR and IR experiments.
- Bergey DH (1994) In: Bergey’s Manual of Determinative Bacteriology, 9th ed., Holt JG, Krieg NR, Sneath PA, eds. (Baltimore: Williams & Wilkins)Google Scholar
- Hoshino T, Kondo T, Uchiyama T, Ogasawara N (1987) Biosynthesis of violacein: a novel rearrangement in tryptophan metabolism with 1, 2-shift of the indole ring. Agric Biol Chem 51, 965–968Google Scholar
- Laatsch H, Thomson RH, Cox PJ (1984) Spectroscopic properties of violacein and related compounds: crystal structure of tetramethylviolacein. J Chem Soc Perkin Trans II 1331–1339Google Scholar
- MacCarthy SA, Sakata T, Kakimoto D, Johnson RM (1985) Production and isolation of purple pigment by Alteromonas luteoviolacea. Bull Japan Soc Sci Fish 51, 479–484Google Scholar
- Margalith PZ (1992) Pigment Microbiology (London: Chapman & Hall)Google Scholar
- Taga N (1968) Some ecological aspects of marine bacteria in the Kuroshio current. Bull Misaki Mar Biol Inst Kyoto Univ 12, 65–76Google Scholar
- Yada S, Ohba M, Enomoto K (2003) Analysis of bacterial species in the Muroto deep seawater. Deep Ocean Water Res (in Japanese) 4, 47–56Google Scholar
- Zimmermann K, von Lengerken J (1979) Analytical methods for nitrate and nitrite determination in foods. 3. Spectrophotometric determination of nitrate and nitrite using sulphanilic acid/1-naphylamine, and of nitrite using resorcinol/zirconium (IV) oxychloride. Nahrung (in German) 23, 929–934CrossRefGoogle Scholar