Molecular Diet Analysis of Phyllosoma Larvae of the Japanese Spiny Lobster Panulirus japonicus (Decapoda: Crustacea)
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To clarify the natural diet of phyllosoma larvae of the Japanese spiny lobster Panulirus japonicus, the sources of 18S rDNA clones obtained from the hepatopancreas were investigated. Of a total of 1537 clones examined, 160 had different restriction profiles from the host larvae, in which 21 restriction types were observed. Nucleotide sequences of 16 of 21 restriction types were successfully determined and their assignments were investigated by homology search and phylogenetic analysis. From seven late-stage larvae collected in spring to early summer, eukaryote DNA molecules of Teleostei, Oomycetes, Mycetozoa, and Fungi were identified. Exogenous DNA from four younger phyllosoma larvae collected in late autumn could not be recovered. A previous study identified DNAs of cnidarians and urochordates in late-stage phyllosoma larvae of a closely related species collected in winter. This indicates that the phyllosoma larvae are opportunistic carnivores, whose diets correlate with the relative abundance of prey organisms in the ambient water.
Keywords18S rDNA natural prey opportunistic carnivore Panulirus japonicus phyllosoma larvae
We thank K. Konishi, National Research Institute of Aquaculture, for valuable information on taxonomy and ecology of lobster larvae. We thanks H. Yamada and K. Satoh, National Research Institute of Far Seas Fisheries, T. Yoshimura, Seikai National Fisheries Research Institute, and the members of RV Shoyo-Maru and RV Shunyo-Maru, for assistance with the sample collection. We also thank H. Hasegawa and M. Michibayashi for their superb technical assistance in DNA analyses. This work was supported in part by grants from the Japanese Society for the Promotion of Science, the Ministry of Agriculture, Forestry, and Fisheries of Japan, and a Grant-in-Aid for Scientific Research on Priority Areas (B) (No. 15380137) from the Ministry of Education, Science, Sports and Culture.
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