Marine Biotechnology

, Volume 10, Issue 1, pp 49–55 | Cite as

Molecular Diet Analysis of Phyllosoma Larvae of the Japanese Spiny Lobster Panulirus japonicus (Decapoda: Crustacea)

  • Nobuaki Suzuki
  • Kouichi Hoshino
  • Keisuke Murakami
  • Haruko Takeyama
  • Seinen Chow
Original Article

Abstract

To clarify the natural diet of phyllosoma larvae of the Japanese spiny lobster Panulirusjaponicus, the sources of 18S rDNA clones obtained from the hepatopancreas were investigated. Of a total of 1537 clones examined, 160 had different restriction profiles from the host larvae, in which 21 restriction types were observed. Nucleotide sequences of 16 of 21 restriction types were successfully determined and their assignments were investigated by homology search and phylogenetic analysis. From seven late-stage larvae collected in spring to early summer, eukaryote DNA molecules of Teleostei, Oomycetes, Mycetozoa, and Fungi were identified. Exogenous DNA from four younger phyllosoma larvae collected in late autumn could not be recovered. A previous study identified DNAs of cnidarians and urochordates in late-stage phyllosoma larvae of a closely related species collected in winter. This indicates that the phyllosoma larvae are opportunistic carnivores, whose diets correlate with the relative abundance of prey organisms in the ambient water.

Keywords

18S rDNA natural prey opportunistic carnivore Panulirus japonicus phyllosoma larvae 

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Nobuaki Suzuki
    • 1
  • Kouichi Hoshino
    • 2
  • Keisuke Murakami
    • 3
  • Haruko Takeyama
    • 4
  • Seinen Chow
    • 5
  1. 1.Ishigaki Tropical StationSeikai National Fisheries Research InstituteOkinawaJapan
  2. 2.Seikai National Fisheries Research InstituteNagasakiJapan
  3. 3.Minami-izu Sea-Farming CenterShizuokaJapan
  4. 4.Tokyo University of Agriculture and Technology, Naka-choKoganeiJapan
  5. 5.National Research Institute of Fisheries ScienceYokosukaJapan

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