Marine Biotechnology

, Volume 9, Issue 6, pp 733–746 | Cite as

Dynamic Regulation of Fluorescent Proteins from a Single Species of Coral

  • Hung-Teh Kao
  • Shelby Sturgis
  • Rob DeSalle
  • Julia Tsai
  • Douglas Davis
  • David F. Gruber
  • Vincent A. Pieribone
Original Article

Abstract

To gain a better understanding of the natural function of fluorescent proteins, we have undertaken quantitative analyses of these proteins in a single species of coral, Montastraea cavernosa, residing around Turneffe atoll, on the Belizean Barrier Reef. We identified at least 10 members of a fluorescent protein family in this species, which consist of 4 distinct spectral classes. As much as a 10-fold change in the overall expression of fluorescent proteins was observed from specimen to specimen, suggesting that fluorescent proteins are dynamically regulated in response to environmental or physiological conditions. We found that the expression of some proteins was inversely correlated with depth, and that groups of proteins were coordinately expressed. There was no relationship between the expression of fluorescent proteins and the natural coloration of the Montastraea cavernosa specimens in this study. These findings have implications for current hypotheses regarding the properties and natural function of fluorescent proteins.

Keywords

barrier reef Belize coordinate expression depth Montastraea cavernosa  real-time quantitative PCR 

Supplementary material

10126_2007_9025_MOESM1_ESM.doc (55 kb)
Supplementary Table 1List of known GFP homologs to the Fluorescent Proteins Derived from Monastraea cavernosa from Belize (DOC 56 kb)

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Hung-Teh Kao
    • 1
    • 2
  • Shelby Sturgis
    • 2
  • Rob DeSalle
    • 3
  • Julia Tsai
    • 4
  • Douglas Davis
    • 5
  • David F. Gruber
    • 6
  • Vincent A. Pieribone
    • 5
  1. 1.Department of Psychiatry and Human Behavior, Division of Biology and Medicine, Warren Alpert Medical SchoolBrown UniversityProvidenceUSA
  2. 2.Nathan Kline Institute and Department of PsychiatryNew York University School of MedicineOrangeburgUSA
  3. 3.Division of Invertebrate ZoologyAmerican Museum of Natural HistoryNew YorkUSA
  4. 4.Skirball Institute Program of Molecular NeurobiologyNew York University School of MedicineNew YorkUSA
  5. 5.John B. Pierce Laboratory, Cellular and Molecular PhysiologyYale UniversityNew HavenUSA
  6. 6.Institute of Marine and Coastal SciencesRutgers UniversityNew BrunswickUSA

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