Marine Biotechnology

, Volume 9, Issue 4, pp 479–490

Protective Effect of Enzymatic Extracts from Microalgae Against DNA Damage Induced by H2O2

  • Rohan Karawita
  • Mahinda Senevirathne
  • Yasantha Athukorala
  • Abu Affan
  • Young-Jae Lee
  • Se-Kwon Kim
  • Joon-Baek Lee
  • You-Jin Jeon
Original Article

Abstract

The enzymatic extracts from seven species of microalgae (Pediastrum duplex, Dactylococcopsis fascicularis, Halochlorococcum porphyrae, Oltmannsiellopsis unicellularis, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis) collected from three habitats (freshwater, tidal pool, and coastal benthic) at Jeju Island in Korea were investigated for their antioxidant activity. Of the extracts tested, the AMG 300 L (an exo 1, 4-α-d-glucosidase) extract of P. duplex, the Viscozyme extract of Navicula sp., and the Celluclast extract of A. longipes provided the most potential as antioxidants. Meanwhile, the Termamyl extract of P. duplex in an H2O2 scavenging assay exhibited an approximate 60% scavenging effect. In this study, we report that the DNA damage inhibitory effects of P. duplex (Termamyl extract) and D. fascicularis (Kojizyme extract) were nearly 80% and 69% respectively at a concentration of 100 μg/ml. Thus, it is suggested that the microalgae tested in this study yield promising DNA damage inhibitory properties on mouse lymphoma L 5178 cells that are treated with H2O2. Therefore, microalgae such as P. duplex may be an excellent source of naturally occurring antioxidant compounds with potent DNA damage inhibition potential.

Keywords

antioxidant activity DNA damage enzymatic extracts H2O2 microalgae  scavenging activity 

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Rohan Karawita
    • 1
  • Mahinda Senevirathne
    • 2
  • Yasantha Athukorala
    • 1
  • Abu Affan
    • 3
  • Young-Jae Lee
    • 4
  • Se-Kwon Kim
    • 5
  • Joon-Baek Lee
    • 3
  • You-Jin Jeon
    • 1
    • 6
  1. 1.Faculty of Applied Marine ScienceCheju National UniversityJejuSouth Korea
  2. 2.Department of Food Science and EngineeringCheju National UniversityJejuSouth Korea
  3. 3.Department of OceanographyCheju National UniversityJejuSouth Korea
  4. 4.Deparment of Veterinary ScienceCheju National UniversityJejuSouth Korea
  5. 5.Department of ChemistryPukyong National UniversityBusanSouth Korea
  6. 6.Radiological Science Research InstituteCheju National UniversityJejuSouth Korea
  7. 7.Korea Ocean Research and Development Institute (KORDI)SeoulRepublic of Korea

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