Marine Biotechnology

, Volume 8, Issue 6, pp 654–662 | Cite as

Heavy Metal-Induced Differential Gene Expression of Metallothionein in Javanese Medaka, Oryzias javanicus

  • Seonock Woo
  • Seungshic Yum
  • Jee Hyun Jung
  • Won Joon Shim
  • Chang-Hoon Lee
  • Taek-Kyun Lee
Original Article

Abstract

A metallothionein (MT) gene was isolated for the first time from Javanese medaka, Oryzias javanicus, which shows high adaptability from freshwater to seawater. The full-length cDNA of MT from O. javanicus (OjaMT) comprises 349 bp, excluding the poly(A)+ stretch, and codes for a total of 60 amino acids. The positions of cysteine residues are highly conserved. The pattern of OjaMT expression induced by six heavy metals was analyzed via real-time quantitative polymerase chain reaction (PCR). The level of hepatic OjaMT mRNA was increased in a dose-dependent manner by Ag, Cd, Cu, and Zn after 24 h of exposure. However, after Cr and Ni exposure, a significant decrease in OjaMT levels was observed. Cadmium-induced OjaMT expression was detectable in fishes as young as 3 months. After Cd exposure, OjaMT induction was prominent in intestine and liver and moderate in muscle and gill. OjaMT mRNA levels could represent a good biomarker for monitoring heavy metals in seawater.

Keywords

heavy metals Javanese medaka metallothionein OjaMT Oryzias javanicus real-time quantitative PCR 

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Seonock Woo
    • 1
  • Seungshic Yum
    • 1
  • Jee Hyun Jung
    • 1
  • Won Joon Shim
    • 1
  • Chang-Hoon Lee
    • 1
  • Taek-Kyun Lee
    • 1
  1. 1.Southern Coastal Environment Research Division, Korea Ocean Research and Development InstituteGeojeKorea

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