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Marine Biotechnology

, Volume 8, Issue 5, pp 481–490 | Cite as

Cloning and Sequencing Analysis of Alginate Lyase Genes from the Marine Bacterium Vibrio sp. O2

  • Hitoshi Kawamoto
  • Akio Horibe
  • Yasunari Miki
  • Takayuki Kimura
  • Katsunori Tanaka
  • Tsuyoshi Nakagawa
  • Makoto Kawamukai
  • Hideyuki Matsuda
Original Article

Abstract

We isolated a new marine bacteria, which displayed alginate-depolymerizing activity in plate assays, from seawater in Mihonoseki Harbor, Japan. Analysis of the 16S ribosomal RNA gene sequence of one of the isolates proved that this alginate-depolymerizing bacterium belonged to the genus Vibrio and it was named Vibrio sp. O2. The alginate lyase genes of Vibrio sp. O2 were cloned and expressed in Escherichia coli. Two alginate lyase-producing clones, pVOA-A4 and pVOA-B5, were obtained. The alginate lyase gene alyVOA from pVOA-A4 was composed of an 858-bp open reading frame (ORF) encoding 285 amino acid residues, while alyVOB from pVOA-B5 was composed of an 828-bp ORF encoding 275 amino acid residues. The degree of identity between the deduced amino acid sequences of AlyVOA or AlyVOB and Photobacterium sp. ATCC43367 alginate poly(ManA)lyase AlxM was 92.3% or 32.6%, respectively. Alginate lyase consensus regions corresponding to the sequences YFKAGXYXQ and RXELR were observed in all three of these sequences. AlyVOA and AlyVOB both degraded polymannuronate in plate assays and were therefore confirmed to be poly(β-D-mannuronate)lyases.

Keywords

Alginate lyase poly(β-D-mannuronate)lyase Vibrio sp. O2 

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Hitoshi Kawamoto
    • 1
    • 2
  • Akio Horibe
    • 2
  • Yasunari Miki
    • 1
  • Takayuki Kimura
    • 1
  • Katsunori Tanaka
    • 2
  • Tsuyoshi Nakagawa
    • 3
  • Makoto Kawamukai
    • 2
  • Hideyuki Matsuda
    • 2
  1. 1.Marine Products Kimuraya Co., Ltd.TottoriJapan
  2. 2.Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental ScienceShimane UniversityMatsueJapan
  3. 3.Center for Integrated Research in ScienceShimane UniversityMatsueJapan

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