Marine Biotechnology

, Volume 8, Issue 4, pp 386–397 | Cite as

A Preliminary Genetic Linkage Map of the Pacific Abalone Haliotis discus hannai Ino

  • Xiande Liu
  • Xiao Liu
  • Ximing Guo
  • Qikang Gao
  • Hongen Zhao
  • Guofan Zhang
Original Article

Abstract

Preliminary genetic linkage maps were constructed for the Pacific abalone (Haliotis discus hannai Ino) using amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA (RAPD), and microsatellite markers segregating in a F1 family. Nine microsatellite loci, 41 RAPD, and 2688 AFLP markers were genotyped in the parents and 86 progeny of the mapping family. Among the 2738 markers, 384 (including 365 AFLP markers, 10 RAPD markers, and 9 microsatellite loci) were polymorphic and segregated in one or both parents: 241 in the female and 146 in the male. The majority of these markers, 232 in the female and 134 in the male, segregated according to the expected 1:1 Mendelian ratio (α = 0.05). Two genetic linkage maps were constructed using markers segregating in the female or the male parent. The female framework map consisted of 119 markers in 22 linkage groups, covering 1773.6 cM with an average intermarker space of 18.3 cM. The male framework map contained 94 markers in 19 linkage groups, spanning 1365.9 cM with an average intermarker space of 18.2 cM. The sex determination locus was mapped to the male map but not to the female map, suggesting a XY-male determination mechanism. Distorted markers showing excess of homozygotes were mapped in clusters, probably because of their linkage to a gene that is incompatible between two parental populations.

Keywords

Amplified fragment length polymorphism (AFLP) genetic linkage map Haliotis discus hannai Ino microsatellite randomly amplified polymorphic DNA (RAPD) sex determination 

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Xiande Liu
    • 1
    • 2
  • Xiao Liu
    • 1
  • Ximing Guo
    • 3
  • Qikang Gao
    • 4
  • Hongen Zhao
    • 5
  • Guofan Zhang
    • 1
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina
  3. 3.Haskin Shellfish Research Laboratory, Institute of Marine and Coastal SciencesRutgers UniversityPort NorrisUSA
  4. 4.Zhejiang UniversityHangzhouChina
  5. 5.Dalian Institute of FisheriesDalianChina

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