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Marine Biotechnology

, Volume 8, Issue 3, pp 246–259 | Cite as

Colonization of the Gastrointestinal Tract of the Farmed South African Abalone Haliotis midae by the Probionts Vibrio midae SY9, Cryptococcus sp. SS1, and Debaryomyces hansenii AY1

  • Brett M. Macey
  • Vernon E. Coyne
Original Article

Abstract

Viable cell counts and/or in situ hybridization were used to determine whether the probionts Vibrio midae SY9, Cryptococcus sp. SS1, and Debaryomyces hansenii AY1 can colonize the gastrointestinal tract of the South African abalone Haliotis midae. The number of culturable probiotic cells reisolated from H. midae fed probiotic-supplemented feed for 3 weeks ranged from 106 to 107 cfu/g gut material. A significant decrease (P < 0.05) in probiont numbers 2 days after feeding the probiotic-supplemented feed had been halted correlated with a significant decrease (P < 0.05) in intestinal protease and amylase activity. There was a positive correlation between Cryptococcus sp. SS1 and amylase activity (r2 = 0.681) and V. midae SY9.8 and protease activity (r2 = 0.711) in the H. midae intestine. Although culturable probionts were isolated from abalone that had not been fed probiotic-supplemented feed for a 2-week period, the drop in the number of probiotic cells colonizing the abalone digestive tract 2 days after feeding with the probiotic-supplemented feed had been halted indicates that farmed abalone should be fed probiotic-supplemented feed at least every second day for maximum benefit.

Keywords

abalone amylase colonization in situ hybridization probiotics protease 

Notes

Acknowledgments

The authors thank Sea Plant Products and Hermanus Abalone for provision of abalone and Di James for conducting the sequencing reactions. The authors acknowledge funding from the Abalone Farmers Association of Southern Africa, Technology and Human Resource for Industry Programme (Grant No. 1608), National Research Foundation (Grant No. 2053565), the Department of Environmental Affairs and Tourism, and the University of Cape Town Research Council.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Department of Molecular and Cell BiologyUniversity of Cape TownRondeboschSouth Africa
  2. 2.Hollings Marine LaboratoryCharlestonUSA

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