Marine Biotechnology

, Volume 6, Issue 5, pp 485–492 | Cite as

Fish as Bioreactors: Transgene Expression of Human Coagulation Factor VII in Fish Embryos

  • Gyulin Hwang
  • Ferenc Müller
  • M. Aziz. Rahman
  • Darren W. Williams
  • Paul J. Murdock
  • K. John Pasi
  • Geoffrey Goldspink
  • Hamid Farahmand
  • Norman Maclean
Article

Abstract

A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.

Keywords

human coagulation factor VII transgenic fish bioreactor. 

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Gyulin Hwang
    • 1
  • Ferenc Müller
    • 1
    • 4
  • M. Aziz. Rahman
    • 1
    • 5
  • Darren W. Williams
    • 1
    • 6
  • Paul J. Murdock
    • 2
  • K. John Pasi
    • 2
  • Geoffrey Goldspink
    • 3
  • Hamid Farahmand
    • 1
  • Norman Maclean
    • 1
  1. 1.Division of Cell SciencesSchool of Biological Sciences, University of SouthamptonSouthamptonU.K.
  2. 2.Haemophilia and Haemostasis CentreRoyal Free and UCL Medical SchoolLondonU.K.
  3. 3.Department of SurgeryRoyal Free and UCL Medical SchoolLondonU.K.
  4. 4.Institute of Toxicology and GeneticsForschungszentrum KarlsruheKarlsruheGermany
  5. 5.School of Chemical and Life SciencesUniversity of Greenwich at MedwayChathamU.K.
  6. 6.Department of BiologyUniversity of WashingtonSeattleU.S.A.

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