Marine Biotechnology

, Volume 6, Issue 1, pp 60–66 | Cite as

Multiple Paternity in Norway Lobster (Nephrops norvegicus L.) Assessed with Microsatellite Markers



We investigated genetic diversity and the mating system of the Norway lobster (Nephrops norvegicus) in a wild population off the Portuguese coast. Approximately 100 individuals were screened for 2 microsatellite loci. For 11 ovigerous lobsters both the female and a sample of her offspring (24 eggs) were genotyped. High genetic diversity was observed for the 2 markers in the population. Paternity within broods was analyzed by comparing multilocus genotypes of each egg with the corresponding mother, and the male parent contribution was then deduced. Multiple paternity was observed in 6 of the 11 broods studied. In those cases, 2 to 3 male parents were likely to have contributed to the fertilization of the eggs. When multiple paternity was involved, the comparative reproductive success of the male parents was quite even. This is the first report of multiple paternity in the Norway lobster. Comparisons with other taxa are presented, and consequences of multiple paternity are discussed.


nephrops microsatellites paternity multiple paternity 


  1. 1.
    Atema, J., Jacobson, S., Karnofsky, E., Olezko-Szuts, S., Stein, L. 1979Pair C3SP formation in the lobster, Homarus americanus: behavioral development, pheromones and mating.Mar Behav Physiol6277296Google Scholar
  2. 2.
    Coombs, N.J., Gough, A.C., Primrose, J.N. 1999Optimisation of DNA and RNA extraction from archival formalin-fixed tissue.Nucleic Acids Res27e12CrossRefPubMedGoogle Scholar
  3. 3.
    Emery, A.M., Wilson, I.J., Craig, S., Boyle, P.R., Noble, L.R. 2001Assignment of paternity groups without access to parental genotypes: multiple mating and developmental plasticity in squid.Mol Ecol1012651278CrossRefPubMedGoogle Scholar
  4. 4.
    Farmer, A.S.D. 1974Reproduction in Nephrops norvegicus (Decapoda: Nephropidae).J Zool Proc Zool Soc Lond174A161183Google Scholar
  5. 5.
    Fjerdingstad, E.J., Boomsma, J.J., Thorén, P. 1998Multiple paternity in the leafcutter ant Atta colombica—a microsatellite DNA study.Heredity80118126CrossRefGoogle Scholar
  6. 6.
    Gibbs, H.L., Weatherhead, P.J. 2001Insights into population ecology and sexual selection in snakes through the application of DNA-based genetic markers.J Hered92173179CrossRefPubMedGoogle Scholar
  7. 7.
    Herbinger, C.M., Doyle, R.W., Taggart, C.T., Lochman, S.E., Brooker, A.L., Wright, J.M., Cook, D. 1997Family relationships and effective population size in natural cohort of Atlantic cod (Gadus morhua) larvae.Can J Fish Aquat Sci541118CrossRefGoogle Scholar
  8. 8.
    Hughes, M., Prodöhl, P., Apostolidis, A., Farestveit, E., Ferguson, A., Heath, P., Jørstad, K., Katsares, V., Kelly, E., Mercer, J., Triantafyllidis, A., and Triantaphyllidis, C. (2001). The GEL project: paternity assessment and breeding structure in the European lobster Homarus gammarus. Presented at the Conference Life Histories, Assessment and Management of Crustacean Fisheries, A Coruña, Spain, October 8–11, 2001.Google Scholar
  9. 9.
    Jennions, M.D., Petrie, M. 2000Why do females mate multiply? A review of the genetic benefits.Biol Rev Camb Philos Soc752164CrossRefPubMedGoogle Scholar
  10. 10.
    Kichler, K., Holder, M.T., Davis, S.K., Márquez, R., Owens, D.W. 1999Detection of multiple paternity in the Kemp’s ridley sea turtle with limited sampling.Mol Ecol8819830CrossRefGoogle Scholar
  11. 11.
    Lebas, N.R. 2001Microsatellite determination of male reproductive success in a natural population of the territorial ornate dragon lizard, Ctenophorus ornatus.Mol Ecol1093203CrossRefGoogle Scholar
  12. 12.
    Levitan, D.R., Sewell, M.A. 1998Fertilization success in free-spawning marine invertebrates: review of the evidence and fisheries implications.Can Spec Publ Fish Aquat Sci125159164Google Scholar
  13. 13.
    McCracken, G.F., Burghardt, G.M., Houts, S.E. 1999Microsatellite markers and multiple paternity in the garter snake Thamnophis sirtalis.Mol Ecol814751479CrossRefPubMedGoogle Scholar
  14. 14.
    Moran, P., Garcia-Vazquez, E. 1998Multiple paternity in Atlantic salmon: a way to maintain genetic variabilty in relicted populations.J Hered89551553CrossRefGoogle Scholar
  15. 15.
    Nelson, K., Hedgecock, D. 1977Electrophoretic evidence of multiple paternity in the lobster Homarus americanus (Milne Edwards).Am Nat111361365CrossRefGoogle Scholar
  16. 16.
    Paul, A.J. 1984Mating frequency and viability of stored sperm in the tanner crab Chionoecetes bairdi (Decapoda, Majidae).J Crust Biol4375381Google Scholar
  17. 17.
    Raymond, M., Rousset, F. 1995GENEPOP (Version 1.2): Population genetics software for exact tests and eucumenism.J Hered86248249Google Scholar
  18. 18.
    Sambrook, J., Fritsch, E., Maniatis, T. 1989Molecular Cloning: A Laboratory Manual.Cold Spring Harbor Laboratory.Cold Spring Harbor, N.Y.Google Scholar
  19. 19.
    Say, L., Pontier, D., Natoli, E. 1999High variation in multiple paternity of domestic cats (Felis catus L.) in relation to environmental conditions.Proc R Soc Lond B Biol Sci26620712074CrossRefPubMedGoogle Scholar
  20. 20.
    Stockley, P., Searle, J.B., MacDonald, D.W., Jones, C.S. 1993Female multiple mating behaviour in the common shrew as a strategy to reduce inbreeding.Proc R Soc Land B Biol Sci254173179Google Scholar
  21. 21.
    Streiff, R., Guillemaud, T., Alberto, F., Magalhães, J., Castro, M., Cancela, M.L. 2001Isolation and characterization of microsatellite loci in the Norway lobster (Nephrops norvegicus).Mol Ecol Notes17172Google Scholar
  22. 22.
    Sugg, D.W., Chesser, R.K. 1994Effective population sizes with multiple paternity.Genetics13711471155Google Scholar
  23. 23.
    Valenzuela, N. 2000Multiple paternity in side-neck turtles Podocnemis expansa: evidence from microsatellite DNA data.Mol Ecol999105CrossRefPubMedGoogle Scholar
  24. 24.
    Waddy, S.L., Aiekn, D.E. 1990Intermolt insemination, an alternative mating strategy for the American lobster (Homarus americanus).Can J Fish Aquat Sci4724922406Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Center of Marine SciencesCCMAR, University of Algarve, P-8005-139 FaroPortugal

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