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Marine Biotechnology

, Volume 4, Issue 3, pp 247–255 | Cite as

Functional Analysis of the Proximal Promoter Regions of Fish Rhodopsin and Myf-5 Genes Using Transgenesis

  • Tzu-Ming  Wang
  • Yau-Hung  Chen
  • Chia-Feng  Liu
  • Huai-Jen  Tsai

Little is known about the cis-acting elements controlling transcriptional activities of fish rhodopsin and myf-5 genes. Transgenic medaka and zebrafish were used to characterize promoters of carp (Cyprinus carpio) rhodopsin gene (cRh) and zebrafish (Danio rerio) myf-5 gene (myf-5), respectively. Transgenic medaka carrying different lengths of cRh upstream fragments fused with enhanced green fluorescence protein (EGFP) gene revealed several functional regions. Both upstream regions from ?1261 to ?163 bp (?1261/?163) and ?163/?138 contributed to enhance the retina-specific activities of cRh. The cNRE (?75/?63) and CSE (?52/?46) motifs located at the cRh proximal promoter were sufficient to drive the transgene expressed in retinae. The ?73/?68 within cNRE was also proved to be essential for the integrity of the minimal cis-regulatory elements. Regarding the myf-5, transgene expression in zebrafish embryos injected with different deletion fragments of zebrafish myf-5 upstream sequences showed that the ?2776/?2456 and ?1046/?844 regions contributed to the enhancer function. The ?290/?154 region might be involved in controlling the translocation of progenitor muscle cells. Moreover, the upstream ?82/?1 region was identified as a minimal promoter required for somite-specific expression. The ?82/?62 region was the most critical sequence for myf-5 specificity.

Keywords

Green Fluorescence Protein Enhance Green Fluorescence Protein Zebrafish Embryo Functional Region Proximal Promoter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2002

Authors and Affiliations

  • Tzu-Ming  Wang
    • 1
  • Yau-Hung  Chen
    • 1
  • Chia-Feng  Liu
    • 1
  • Huai-Jen  Tsai
    • 1
  1. 1.Institute of Fisheries Science, National Taiwan University, 1, Sec. 4, Roosevelt Road, Taipei, Taiwan 106, Republic of ChinaCN

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