Marine Biotechnology

, Volume 4, Issue 2, pp 127–131

In Situ Microspatial Imaging Using Two-Photon and Confocal Laser Scanning Microscopy of Bacteria and Extracellular Polymeric Secretions (EPS) Within Marine Stromatolites

  • Tomohiro Kawaguchi
  • Alan W. Decho

DOI: 10.1007/s10126-001-0073-7

Cite this article as:
Kawaguchi, T. & Decho, A. Mar. Biotechnol. (2002) 4: 127. doi:10.1007/s10126-001-0073-7


The combination of a hydrophilic embedding resin, Nanoplast, with fluorescent probes, and subsequent imaging using two-photon and confocal laser scanning microscopy (2P-LSM and CLSM) has allowed in imaging of the in situ microspatial arrangements of microbial cells and their extracellular polymeric secretion (EPS) within marine stromatolites. Optical sectioning by 2P-LSM and CLSM allowed imaging of endolithic cyanobacteria cells, Solentia sp., seen within carbonate sand grains. 2P-LSM allowed very clear imaging with a high resolution of bacteria using DAPI, which normally require UV excitation and reduced photo-bleaching of fluorescent probes.

Key words: confocal laser scanning microscopy, Schizothrix sp., Solentia sp., extracellular polymeric secretion, marine stromatolite, two-photon laser scanning microscopy. 

Copyright information

© 2002 Springer-Verlag New York Inc.

Authors and Affiliations

  • Tomohiro Kawaguchi
    • 1
  • Alan W. Decho
    • 1
  1. 1.Department of Environmental Health Sciences, School of Public Health, University of South Carolina, Columbia, SC 29208, U.S.A.US

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