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Marine Biotechnology

, Volume 3, Issue 5, pp 454–462 | Cite as

Characterization of Psychrotrophic Bacteria in the Surface and Deep-Sea Waters from the Northwestern Pacific Ocean Based on 16S Ribosomal DNA Analysis

  • Ocky Karna  Radjasa
  • Hidetoshi  Urakawa
  • Kumiko  Kita-Tsukamoto
  • Kouichi  Ohwada

Abstract:

Seventy-eight 4°C-culturable bacteria were isolated using ZoBell 2216E medium from surface (0–200 m) and deep-sea (1000–9671 m) waters in the northwestern Pacific Ocean. Growth studies indicated that all 4°C-culturable bacteria were psychrotrophs. Six phylotypes were observed in the surface water samples and 8 phylotypes in the deep-sea waters. Phylogenetic characterization based on 16S ribosomal DNA sequence analysis of the representative phylotypes revealed that some bacterial genera, Pseudoalteromonas, Photobacterium, and Vibrio, were common to surface and deep-sea waters, and others, Pseudomonas and Halomonas, specifically occurred in surface water. Overall, the members of Vibrionaceae appear to be dominant in both habitats.

Key words: marine psychrotrophs, 16S rRNA, RFLP, Vibrionaceae, Vibrio, Photobacterium, Pseudoalteromonas. 

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Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Ocky Karna  Radjasa
    • 1
  • Hidetoshi  Urakawa
    • 1
  • Kumiko  Kita-Tsukamoto
    • 1
  • Kouichi  Ohwada
    • 1
  1. 1.Marine Microbiology Division, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, JapanJP

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