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Microbiota dispersion in the Uyuni salt flat (Bolivia) as determined by community structure analyses

  • Cesar A. Pérez-FernándezEmail author
  • Mercedes Iriarte
  • Jessica Rivera-Pérez
  • Raymond L. Tremblay
  • Gary A. Toranzos
Original Article

Abstract

Soil microbial communities are an important component of biological diversity and terrestrial ecosystems which is responsible for processes such as decomposition, mineralization of nutrients, and accumulation of organic matter. One of the factors that provide information on the mechanisms regulating biodiversity is spatial scaling. We characterized the microbial communities using 16S rRNA gene sequences from DNA isolated from halite at various locations and correlated these to geographic distance in the Uyuni salt flat (Bolivia). Sequences from each site were analyzed to determine any spatial patterns of diversity, as well as to describe the microbial communities. Results suggest that different taxa are able to disperse over Uyuni’s surface crust regardless of distance. As expected, ubiquitous taxa included members of Halobacteriaceae such as Haloarcula, Halorubrum, Halorhabdus, Halolamina, and halophilic bacteria Salinibacter, Halorhodospira, and unclassified members of the Gammaproteobacteria. Archaeal communities were homogeneous across the salt flat. In contrast, bacterial communities present strong local variations which could be attributed to external factors. Likely sources for these variations are the Rio Grande river influent in the south shore and the Tunupa volcano influencing the northern area.

Keywords

Hypersaline Microbial dispersion 16S rRNA gene Uyuni salt flat 

Notes

Acknowledgements

We thank Mr DNA Molecular Research Laboratory (Shallowater, TX) for their fast and reliable sequencing services.

Funding information

This study was partially funded by the Research Initiative for Scientific Enhancement Program (Grant No. 5R25GM061151-12, -13) and the support of Universidad Mayor de San Simón’s Dirección de Ciencias y Tecnología (UMSS-DCyT).

Supplementary material

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Rarefaction curves for unnormalized read counts (PNG 173 kb)

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Rare faction curves for normalized read counts (PNG 167 kb)

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High resolution image (TIF 1242 kb)
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Cannonical correspondence analysis of the microbial communities with the physicochemical description of (Risacher and Fritz 1991, 2000) (PNG 211 kb)

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ESM 7 (DOCX 15 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Cesar A. Pérez-Fernández
    • 1
    Email author
  • Mercedes Iriarte
    • 2
  • Jessica Rivera-Pérez
    • 1
    • 3
  • Raymond L. Tremblay
    • 4
    • 5
  • Gary A. Toranzos
    • 1
  1. 1.Environmental Microbiology LaboratoryUniversity of Puerto Rico, Rio Piedras campusSan JuanPuerto Rico
  2. 2.Centro de Aguas y Saneamiento Ambiental, Facultad de Ciencias y TecnologíaUniversidad Mayor de San SimónCochabambaBolivia
  3. 3.Ecosystems and Global ChangeLincolnNew Zealand
  4. 4.Biology DepartmentUniversity of Puerto Rico, Humacao campusHumacaoPuerto Rico
  5. 5.Center for Applied Tropical Ecology and ConservationUniversity of Puerto RicoSan JuanPuerto Rico

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