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International Microbiology

, Volume 22, Issue 1, pp 121–130 | Cite as

Microencapsulation of endophytic LAB (KCC-41) and its probiotic and fermentative potential for cabbage kimchi

  • Chae Eun Song
  • Palaniselvam Kuppusamy
  • Young-IL Jeong
  • Han Hyo ShimEmail author
  • Kyung Dong LeeEmail author
Original Article
  • 62 Downloads

Abstract

The aim of the present study was to isolate novel lactic acid bacteria (LAB) from hairy vetch forage crop and characterize their probiotic and fermentative potential for preparing Korean cabbage kimchi. First, functional characterization of isolated strains such as antagonistic property, auto-aggregation, antibiotic susceptibility, and extracellular enzyme production was performed. The isolated Lactobacillus plantarum KCC-41 strain was able to inhibit pathogenic fungal spore formation. It showed susceptibility to common commercial antibiotics drugs. The selected LAB strain was then subjected to microencapsulation with alginate biopolymer. Its ability to survive in in vitro simulated gastro-intestinal fluid was evaluated. It was also used in the fermentation of cabbage kimchi samples. The encapsulated KCC-41 strain could effectively lead to kimchi fermentation in terms of reducing its pH and dominating bacterial count. It also significantly increased organic acid production than non-encapsulated LAB (KCC-41) for cabbage kimchi samples.

Keywords

Lactobacillus sp. Microencapsulation Gastro-intestinal tolerance Cabbage kimchi Fermentation quality 

Abbreviations

LAB

lactic acid bacteria

HPLC

high-performance liquid chromatography

SCFA

short-chain fatty acids

MRS

De Man, Rogosa, and Sharpen agar

CFU

colony forming unit

IRG

Italian rye grass

SGF

simulated gastric juice

SIF

simulated intestinal juice

Notes

Acknowledgements

We are thankful to Prof. Dr. K.C. Choi, NIAS, Korea, for the gifted novel Lactobacillus strains which is isolated from forage sources.

Authors’ Contributions

KDL designed the research; CES, PK, and YIJ performed the experiments. KDL, PK, CES, HHS, and YIL analyzed the data and PK and KDL wrote the paper. All authors read and approved the final manuscript.

Compliance with ethical standards

Consent for publication

Not applicable.

Ethical approval and consent to participate

Not applicable.

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

10123_2018_34_MOESM1_ESM.docx (7.1 mb)
ESM 1 (DOCX 7230 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Lifelong Education CenterChonnam National UniversityKwangjuRepublic of Korea
  2. 2.Grassland and Forage Division, National Institute of Animal ScienceRural Development AdministrationCheonanRepublic of Korea
  3. 3.Biomedical Research InstitutePusan National University HospitalBusanRepublic of Korea
  4. 4.Department of BiotechnologySunchon National UniversitySuncheonRepublic of Korea
  5. 5.Department of Oriental Medicine MaterialsDongsin UniversityNajuRepublic of Korea

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