JMJD2A sensitizes gastric cancer to chemotherapy by cooperating with CCDC8

  • Tadahiko Nakagawa
  • Yasushi SatoEmail author
  • Toshihito Tanahashi
  • Yasuhiro Mitsui
  • Yoshifumi Kida
  • Yasuteru Fujino
  • Misato Hirata
  • Shinji Kitamura
  • Hiroshi Miyamoto
  • Koichi Okamoto
  • Naoki Muguruma
  • Yoshimi Bando
  • Tetsuji Takayama
Original Article



Jumonji domain-containing protein 2A (JMJD2A) of the JMJD2 family of histone lysine demethylases has been implicated in tumorigenesis. However, its expression and role in gastric cancer (GC) drug resistance remain unknown. Here, we investigated the role of JMJD2A in GC chemotherapeutic susceptibility and its clinical relevance in GC.


We selected 12 relevant genes from previously identified gene signatures that can predict GC susceptibility to docetaxel, cisplatin, and S-1 (DCS) therapy. Each gene was knocked down using siRNA in GC cell lines, and cell viability assays were performed. JMJD2A expression in GC cell lines and tissues was assessed using qRT-PCR and immunohistochemistry, respectively. A JMJD2A downstream target related to drug susceptibility was examined using whole-gene expression array and immunoprecipitation.


Among the 12 candidate genes, down-regulation of JMJD2A showed the maximum effect on GC susceptibility to anti-cancer drugs and increased the IC50 values for 5-FU, cisplatin, and docetaxel 15.3-, 2.7-, and 4.0-fold, respectively. JMJD2A was universally expressed in 12 GC cell lines, and its overexpression in GC tissue was positively correlated with tumor regression in 34 DCS-treated patients. A whole-gene expression array of JMJD2A-knockdown GC cells demonstrated a significant decrease in the expression of pro-apoptotic coiled-coil domain containing 8 (CCDC8), a downstream target of JMJD2A. Direct interaction between CCDC8 and JMJD2A was verified using immunoprecipitation. CCDC8 inhibition restored drug resistance to docetaxel, cisplatin, and S-1.


Our results indicate that JMJD2A is a novel epigenetic factor affecting GC chemotherapeutic susceptibility, and JMJD2A/CCDC8 is a potential GC therapeutic target.


Gastric cancer Histone lysine demethylases Jumonji domain-containing protein 2A (JMJD2A) Coiled-coil domain containing 8 (CCDC8) Drug resistance 



This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI [Grant number JP16K19348 and JP18K07942]. We thank Hideaki Horikawa for analysis of microarray data, supported by the Support Center for Advanced Medical Sciences, Tokushima University Graduate School of Biomedical Sciences.

Compliance with ethical standards

Conflict of interest

Tetsuji Takayama received a research fund from TAIHO Pharmaceutical Co., Ltd. (Tokyo, Japan).

Human rights statement

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1964 and later versions.

Informed consent

Informed consent or a substitute for it was obtained from all patients for being included in the study.

Supplementary material

10120_2019_1024_MOESM1_ESM.pptx (7.3 mb)
Supplementary file1 (PPTX 7463 kb)


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Copyright information

© The International Gastric Cancer Association and The Japanese Gastric Cancer Association 2019

Authors and Affiliations

  • Tadahiko Nakagawa
    • 1
    • 2
  • Yasushi Sato
    • 3
    Email author
  • Toshihito Tanahashi
    • 1
  • Yasuhiro Mitsui
    • 1
  • Yoshifumi Kida
    • 1
  • Yasuteru Fujino
    • 1
  • Misato Hirata
    • 1
  • Shinji Kitamura
    • 1
  • Hiroshi Miyamoto
    • 1
  • Koichi Okamoto
    • 1
  • Naoki Muguruma
    • 1
  • Yoshimi Bando
    • 4
  • Tetsuji Takayama
    • 1
  1. 1.Department of Gastroenterology and Oncology, Institute of Biomedical SciencesTokushima University Graduate SchoolTokushimaJapan
  2. 2.Department of Health and Nutrition, Faculty of Nursing and NutritionThe University of ShimaneShimaneJapan
  3. 3.Department of Community Medicine for Gastroenterology and Oncology, Institute of Biomedical SciencesTokushima University Graduate School of Biomedical SciencesTokushimaJapan
  4. 4.Division of PathologyTokushima University HospitalTokushimaJapan

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