Fluorescent detection of Cu(II) by chitosan-based AIE bioconjugate
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Detection of Cu(II) is very important in disease diagnose, biological system detection and environmental monitoring. Previously, we found that the product TPE-CS prepared by attaching the chromophores of tetraphenylethylene (TPE) to the chitosan (CS) chains showed excellent fluorescent properties. In this study, we tried to use TPE-CS for detecting Cu(II) because of the stable complexation of CS with heavy metals and the luminosity mechanism of the Restriction of Intramolecular Rotations (RIR) for aggregation-induced emission (AIE)-active materials. The fluorescence intensity changed when TPE-CS was contacted with different metal ions, to be specific, no change for Na+, slightly increase for Hg2+, Pb2+, Zn2+, Cd2+, Fe2+, Fe3+ due to the RIR caused by the complexation between CS and metal ions. However, for Cu2+, an obvious fluorescence decrease was observed because of the Photoinduced-Electron-Transfer (PET). Moreover, we found that the quenched FL intensity of TPE-CS was proportional to the concentration of Cu(II) in the range of 5 μmol/L to 100 μmol/L, which provided a new way to quantitatively detect Cu(II). Besides, TPE-CS has excellent water-solubility as well as absorbability (the percentage of removal, R = 84%), which is an excellent detection probe and remover for Cu(II).
KeywordsAggregation-induced emission Chitosan Cu(II) Fluorescent detection
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