In this study we investigated the influence of Photofrin-based photodynamic therapy (PDT) on the migration of two human glioma cell lines in vitro. U87 and U25ln tumour cells were treated with Photofrin at various doses and subjected to a fixed optical (632 nm) dose of 100 mJ/cm2. Photofrin cytotoxicity was determined using MTT and colony forming assays. Using a matrigel artificial basement membrane migration assay, we demonstrated that low doses of subcytotoxic PDT treatment, such as PDT with 2.5 µg/ml Photofrin on U87 cells and 1 µg/ml on U25ln cells, significantly (p<0.001) inhibited in vitro migration of both cell lines. Furthermore, in a qualitative spheroid confrontation assay, subcytotoxic PDT of co-cultures between tumour spheroids and brain aggregates resulted in an absence of progressive tumour invasion and destruction of the brain aggregate. In conclusion, our data indicate that low-dose subcytotoxic PDT with Photofrin significantly inhibits invasiveness of U87 and U25ln cells.
This is a preview of subscription content, log in to check access.
Paper received 8 May 2002; accepted after revision 19 June 2002.
Correspondence to: Michael Chopp, PhD, Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, MI 48202, USA. Tel: 313-916-3936; Fax: 313-916-1318; e-mail: firstname.lastname@example.org
About this article
Cite this article
Jiang, F., Chopp, M., Katakowski, M. et al. Photodynamic Therapy with Photofrin Reduces Invasiveness of Malignant Human Glioma Cells. Lasers Med Sci 17, 280–288 (2002) doi:10.1007/s101030200041
- Keywords: Glioma; In vitro migration; Photodynamic therapy; PDT; Tumour invasion; Tumour migration