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Laser-induced fluorescence diagnosis of stomach tumor

Abstract

The purpose of this study is to demonstrate the capabilities of laser spectral and video fluorescence diagnosis used for stomach tumors using 5-ALA photosensitizer. The spectroscopic method is presented with an example of a characteristic fluorescence spectrum from stomach with 5-ALA and quantitative statistics. The laser excitation wavelength was 632.8 nm. The analysis of the video system is presented with clinical statistics. The penetration depth of 3–4 mm of the He–Ne laser during the spectroscopic study allowed for scanning the mucous and submucous layers of the stomach and for detecting tumorous growths in these layers. Registration of fluorescence using the spectral system enabled surgeons to conduct express estimation of dubious stomach tissues, to make biopsy from doubtful areas to reveal precancer and early cancer states. The video fluorescence analysis with the application of 5-ALA-induced PPIX may be recommended for the use as an express method of diagnosis including early diagnosis of malignant stomach diseases as well as for intraoperative assessment of tumor extension and detection of canceromatous foci during laparoscopy. The optimal time interval for the diagnosis (regardless of the nature of the study – endoscopic, laparoscopic, or intraoperative) is 2–4 h from the administration of photosensitizer. The optimal dose of the photosensitizer is 20 mg per 1 kg of the body weight.

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Funding

This study was funded by the Center for collective use of scientific equipment No. 74834 “Technological and diagnostic center for the production, research and certification of micro and nanostructures” in GPI RAS, and MEPhI Academic Excellence Project (agreement with the Ministry of Education and Science of the Russian Federation of August 27, 2013, project no. 02.a03.21.0005).

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Correspondence to Nina Kalyagina.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the Russian Federation and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Enrolled patients provided written informed consent. The examination was made in accordance with the approved principles. All the preparations and the equipment used are officially certified for the clinical use.

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Informed consent was obtained from all individual participants included in the study.

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Loshchenov, M., Levkin, V., Kalyagina, N. et al. Laser-induced fluorescence diagnosis of stomach tumor. Lasers Med Sci 35, 1721–1728 (2020). https://doi.org/10.1007/s10103-020-02963-x

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Keywords

  • Fluorescence
  • Gastric cancer
  • Spectroscopy
  • 5-ALA
  • Video fluorescence
  • Stomach diagnosis