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Laser-induced fluorescence diagnosis of stomach tumor


The purpose of this study is to demonstrate the capabilities of laser spectral and video fluorescence diagnosis used for stomach tumors using 5-ALA photosensitizer. The spectroscopic method is presented with an example of a characteristic fluorescence spectrum from stomach with 5-ALA and quantitative statistics. The laser excitation wavelength was 632.8 nm. The analysis of the video system is presented with clinical statistics. The penetration depth of 3–4 mm of the He–Ne laser during the spectroscopic study allowed for scanning the mucous and submucous layers of the stomach and for detecting tumorous growths in these layers. Registration of fluorescence using the spectral system enabled surgeons to conduct express estimation of dubious stomach tissues, to make biopsy from doubtful areas to reveal precancer and early cancer states. The video fluorescence analysis with the application of 5-ALA-induced PPIX may be recommended for the use as an express method of diagnosis including early diagnosis of malignant stomach diseases as well as for intraoperative assessment of tumor extension and detection of canceromatous foci during laparoscopy. The optimal time interval for the diagnosis (regardless of the nature of the study – endoscopic, laparoscopic, or intraoperative) is 2–4 h from the administration of photosensitizer. The optimal dose of the photosensitizer is 20 mg per 1 kg of the body weight.

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  1. 1.

    Sitarz R, Skierucha M, Mielko J, Offerhaus GJA, Maciejewski R, Polkowski WP (2018) Gastric cancer: epidemiology, prevention, classification, and treatment. Cancer Manag Res 10:239–248.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  2. 2.

    Waddingham W, Graham D, Banks M, Jansen MF (2018) The evolving role of endoscopy in the diagnosis of premalignant gastric lesions. F1000Research 7;F1000 faculty rev–715 Doi:

  3. 3.

    Kato H (2012) Our experience with photodynamic diagnosis and photodynamic therapy for lung cancer. J Natl Compr Cancer Netw 10(Suppl 2):S3–S8.

    CAS  Article  Google Scholar 

  4. 4.

    Filonenko EV., Kaprin AD, Alekseev BY, Ivanova-Radkevich VI, Slovokhodov EK, Filinov VL, Urlova AN (2017) Fluorescence diagnosis of bladder Cancer with agent Hexasens – the results of multicenter trial. Biomedical photonics 6(1):20-27 (in Russian).

  5. 5.

    Osman E, Alnaib Z, Kumar N (2017) Photodynamic diagnosis in upper urinary tract urothelial carcinoma: a systematic review. Arab J Urol 15(2):100–109.

    Article  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Yonemura Y, Endo Y, Canbay E, Liu Y, Ishibashi H, Mizumoto A, Hirano M, Imazato Y, Takao N, Ichinose M, Noguchi K, Li Y, Wakama S, Yamada K, Hatano K, Shintani H, Yoshitake H, Ogura SI (2017) Photodynamic detection of peritoneal metastases using 5-Aminolevulinic acid (ALA). Cancers (Basel) 9(3):E23.

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Stepp H, Stummer W (2018) 5-ALA in the management of malignant glioma. Lasers Surg Med 50(5):399–419.

    Article  PubMed  Google Scholar 

  8. 8.

    Savelieva TA, Kalyagina N, Kholodtsova M, Potapov A, Goryainov S, Loschenov VB (2012) Numerical modelling and in vivo analysis of fluorescent and laser light backscattered from glial brain tumours SPIE proceedings Vol. 8230, 82300L-1-L9.

  9. 9.

    Novikova T (2017) Optical techniques for cervical neoplasia detection Beilstein. J Nanotechnol 8:1844–1862.

    CAS  Article  Google Scholar 

  10. 10.

    Cieplik F, Buchalla W, Hellwig E, Al-Ahmad A, Hiller KA, Maisch T, Karygianni L (2017) Antimicrobial photodynamic therapy as an adjunct for treatment of deep carious lesions-a systematic review. Photodiagn Photodyn Ther 18:54–62.

    CAS  Article  Google Scholar 

  11. 11.

    Blasi MA, Pagliara MM, Lanza A, Sammarco MG, Caputo CG, Grimaldi G, Scupola A (2018) Photodynamic therapy in ocular oncology. Biomedicines 6(1):E17.

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Kawczyk-Krupka A, Pucelik B, Międzybrodzka A, Sieroń A, Dąbrowski JM (2018) Photodynamic therapy as an alternative to antibiotic therapy for the treatment of infected leg ulcers. Photodiagnosis Photodyn Ther S1572-1000(18):30045–30040.

    CAS  Article  Google Scholar 

  13. 13.

    Bhatta AK, Keyal U, Wang X, Gellén E (2017) A review of the mechanism of action of lasers and photodynamic therapy for onychomycosis. Lasers Med Sci 32(2):469–474.

    Article  PubMed  Google Scholar 

  14. 14.

    Battersby AR, Fookes CJR, Matcham GWJ, Mcdonald E (1980) Biosynthesis of the pigments of life: formation of the macrocycle. Nature 285:17

    CAS  Article  Google Scholar 

  15. 15.

    Shemin D, Russell CS (1953) δ-Aminolevulinic acid, its role in the biosynthesis of Porphyrins and Purines1. J Am Chem Soc 75:4873–4874.

    CAS  Article  Google Scholar 

  16. 16.

    Gomes ATPC, Neves MGPMS, Cavaleiro JAS (2018) Cancer, photodynamic therapy and porphyrin-type derivatives. An Acad Bras Cienc 90(1 Suppl 2):993–1026.

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Namikawa T, Inoue K, Uemura S, Shiga M, Maeda H, Kitagawa H, Fukuhara H, Kobayashi M, Shuin T, Hanazaki K (2014) Photodynamic diagnosis using 5-aminolevulinic acid during gastrectomy for gastric cancer. J Surg Oncol 109(3):213–217.

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Chen W, Jassemnejad B, Crull J, Knobbe E, Nordquist R (1996) Detection and characterization of chemical induced abnormal tissue and rat tumors at different stages using fluorescence spectroscopy Proc. SPIE; 2679-11. doi:

  19. 19.

    Isomoto H, Nanashima A, Senoo T, Ogiwara K, Hashisako M, Ohnita K, Yamaguchi N, Kunizaki M, Hidaka S, Fukuda H, Ishii H, Matsushima K, Minami H, Akazawa Y, Takeshima F, Fukuoka J, Nagayasu T, Nakao K (2015) In vivo fluorescence navigation of gastric and upper gastrointestinal tumors by 5-aminolevulinic acid mediated photodynamic diagnosis with a laser-equipped video image endoscope. Photodiagn Photodyn Ther 12(2):201–208.

    CAS  Article  Google Scholar 

  20. 20.

    Nakamura M, Nishikawa J, Hamabe K, Goto A, Nishimura J, Shibata H, Nagao M, Sasaki S, Hashimoto S, Okamoto T, Sakaida I (2015) Preliminary study of photodynamic diagnosis using 5-aminolevulinic acid in gastric and colorectal tumors. World J Gastroenterol 21(21):6706–6712.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Koizumi N, Harada Y, Minamikawa T, Tanaka H, Otsuji E, Takamatsu T. (2016) Recent advances in photodynamic diagnosis of gastric cancer using 5-aminolevulinic acid. 22(3):1289-96. doi:

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This study was funded by the Center for collective use of scientific equipment No. 74834 “Technological and diagnostic center for the production, research and certification of micro and nanostructures” in GPI RAS, and MEPhI Academic Excellence Project (agreement with the Ministry of Education and Science of the Russian Federation of August 27, 2013, project no. 02.a03.21.0005).

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Correspondence to Nina Kalyagina.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the Russian Federation and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Enrolled patients provided written informed consent. The examination was made in accordance with the approved principles. All the preparations and the equipment used are officially certified for the clinical use.

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Informed consent was obtained from all individual participants included in the study.

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Loshchenov, M., Levkin, V., Kalyagina, N. et al. Laser-induced fluorescence diagnosis of stomach tumor. Lasers Med Sci 35, 1721–1728 (2020).

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  • Fluorescence
  • Gastric cancer
  • Spectroscopy
  • 5-ALA
  • Video fluorescence
  • Stomach diagnosis