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Comparative effects of photobiomodulation therapy at wavelengths of 660 and 808 nm on regeneration of inferior alveolar nerve in rats following crush injury

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Abstract

The aim of the present study was to investigate the therapeutic effects of 660-nm and 880-nm photobiomodulation therapy (PBMT) following inferior alveolar nerve (IAN) crush injury. Following the nerve crush injuries of IAN, 36 Wistar rats were randomly divided into three groups as follows: (1) control, (2) 660-nm PBMT, and (3) 808-nm PBMT (GaAlAs laser, 100 J/cm2, 70 mW, 0.028-cm2 beam). PBMT was started immediately after surgery and performed once every 3 days during the postoperative period. At the end of the 30-day treatment period, histopathological and histomorphometric evaluations of tissue sections were made under a light and electron microscope. The ratio of the inner axonal diameter to the total outer axonal diameter (g-ratio) and the number of axons per square micrometer were evaluated. In the 808-nm PBMT group, the number of nerve fibers with suboptimal g-ratio ranges of 0–0.49 (p < 0.001) is significantly lower than expected, which indicates better rate of myelinization in the 808-nm PBMT group. The number of axons per square micrometer was significantly higher in the 808-nm PBMT group when compared with the control (p < 0.001) and 660-nm PBMT group (p = 0.010). The data and the histopathological investigations suggest that the PBMT with the 808-nm wavelength along with its settings was able to enhance IAN regeneration after nerve crush injury.

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References

  1. 1.

    Bataineh AB (2001) Sensory nerve impairment following mandibular third molar surgery. J Oral Maxillofac Surg 59:1012–1017

  2. 2.

    Ellies LG, Hawker PB (1993) The prevalence of altered sensation associated with implant surgery. Int J Oral Maxillofac Implants 8:674–679

  3. 3.

    Grötz KA, Al-Nawas B, de Aguiar EG et al (1998) Treatment of injuries to the inferior alveolar nerve after endodontic procedures. Clin Oral Investig 2:73–76

  4. 4.

    Haas DA (2006) Articaine and paresthesia: epidemiological studies. J Am Coll Dent 73:5–10

  5. 5.

    McLeod NMH, Bowe DC (2016) Nerve injury associated with orthognathic surgery. Part 2: inferior alveolar nerve. Br J Oral Maxillofac Surg 54:366–371

  6. 6.

    Tay ABG, Bin LJ, Lye KW et al (2015) Inferior alveolar nerve injury in trauma-induced mandible fractures. J Oral Maxillofac Surg 73:1328–1340

  7. 7.

    Sumer M, Bas B, Yıldız L (2007) Inferior alveolar nerve paresthesia caused by a dentigerous cyst associated with three teeth. Med Oral Patol Oral Cir Bucal 12:E388–E390

  8. 8.

    Schultze-Mosgau S, Reich RH (1993) Assessment of inferior alveolar and lingual nerve disturbances after dentoalveolar surgery, and of recovery of sensitivity. Int J Oral Maxillofac Surg 22:214–217

  9. 9.

    Long H, Zhou Y, Liao L et al (2012) Coronectomy vs. total removal for third molar extraction: a systematic review. J Dent Res 91:659–665

  10. 10.

    Leung YY, Fung PP-L, Cheung LK (2012) Treatment modalities of neurosensory deficit after lower third molar surgery: a systematic review. J Oral Maxillofac Surg 70:768–778

  11. 11.

    Alhassani AA, AlGhamdi AST (2010) Inferior alveolar nerve injury in implant dentistry: diagnosis, causes, prevention, and management. J Oral Implantol 36:401–407

  12. 12.

    Angeletti P, Pereira MD, Gomes HC et al (2010) Effect of low-level laser therapy (GaAlAs) on bone regeneration in midpalatal anterior suture after surgically assisted rapid maxillary expansion. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109:e38–e46

  13. 13.

    Wang X, Tian F, Reddy DD et al (2017) Up-regulation of cerebral cytochrome-c-oxidase and hemodynamics by transcranial infrared laser stimulation: a broadband near-infrared spectroscopy study. J Cereb Blood Flow Metab 37:3789–3802

  14. 14.

    Rojas JC, Gonzalez-Lima F (2013) Neurological and psychological applications of transcranial lasers and LEDs. Biochem Pharmacol 86:447–457

  15. 15.

    de Rosso MPO, Buchaim DV, Kawano N et al (2018) Photobiomodulation therapy (PBMT) in peripheral nerve regeneration: a systematic review. Bioengineering 5:44–56

  16. 16.

    Hashmi JT, Huang Y-Y, Osmani BZ et al (2010) Role of low-level laser therapy in neurorehabilitation. PM R 2:S292–S305

  17. 17.

    Coulthard P, Kushnerev E, Jm Y et al (2014) Interventions for iatrogenic inferior alveolar and lingual nerve injury. Cochrane Database Syst Rev 16:CD005293

  18. 18.

    Naftel JP, Richards LP, Pan M, Bernanke JM (1999) Course and composition of the nerves that supply the mandibular teeth of the rat. Anat Rec 256:433–447

  19. 19.

    Germanà G, Muglia U, Santoro M et al (1992) Morphometric analysis of sciatic nerve and its main branches in the rabbit. Biol Struct Morphog 4:11–15

  20. 20.

    Peng Q, Juzeniene A, Chen J et al (2008) Lasers in medicine. Rep Prog Phys 71:1–28

  21. 21.

    Rochkind S, Leider-Trejo L, Nissan M et al (2007) Efficacy of 780-nm laser phototherapy on peripheral nerve regeneration after neurotube reconstruction procedure (double-blind randomized study). Photomed Laser Surg 25:137–143

  22. 22.

    Andreo L, Soldera CB, Ribeiro BG et al (2017) Effects of photobiomodulation on experimental models of peripheral nerve injury. Lasers Med Sci 32:2155–2165

  23. 23.

    Gasperini G, De Siqueira ICR, Costa LR (2014) Lower-level laser therapy improves neurosensory disorders resulting from bilateral mandibular sagittal split osteotomy: a randomized crossover clinical trial. J Cranio-Maxillofacial Surg 42:e130–e133

  24. 24.

    Guarini D, Gracia B, Ramírez-Lobos V et al (2018) Laser biophotomodulation in patients with neurosensory disturbance of the inferior alveolar nerve after sagittal split ramus osteotomy: a 2-year follow-up study. Photomed Laser Surg 36:3–9

  25. 25.

    Alghamdi KM, Kumar A (2012) Low-level laser therapy : a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237–249

  26. 26.

    Barez MM, Tajziehchi M, Heidari MH et al (2017) Stimulation effect of low level laser therapy on sciatic nerve regeneration in rat. J Lasers Med Sci 8:S32–S37

  27. 27.

    Wang CZ, Chen YJ, Wang YH et al (2014) Low-level laser irradiation improves functional recovery and nerve regeneration in sciatic nerve crush rat injury model. PLoS One 9:e103348

  28. 28.

    Takhtfooladi MA, Jahanbakhsh F, Takhtfooladi HA et al (2015) Effect of low-level laser therapy (685 nm, 3 J/cm2) on functional recovery of the sciatic nerve in rats following crushing lesion. Lasers Med Sci 30:1047–1052

  29. 29.

    Takhtfooladi MA, Sharifi D (2015) A comparative study of red and blue light-emitting diodes and low-level laser in regeneration of the transected sciatic nerve after an end to end neurorrhaphy in rabbits. Lasers Med Sci 30:2319–2324

  30. 30.

    Medalha CC, Di Gangi GC, Barbosa CB et al (2012) Low-level laser therapy improves repair following complete resection of the sciatic nerve in rats. Lasers Med Sci 27:629–635

  31. 31.

    Barbosa RI, Marcolino AM, De Jesus Guirro RR et al (2010) Comparative effects of wavelengths of low-power laser in regeneration of sciatic nerve in rats following crushing lesion. Lasers Med Sci 25:423–430

  32. 32.

    de Martins DO, dos Santos FM, de Oliveira ME et al (2013) Laser therapy and pain-related behavior after injury of the inferior alveolar nerve: possible involvement of neurotrophins. J Neurotrauma 30:480–486

  33. 33.

    de Martins DO, dos Santos FM, Ciena AP et al (2017) Neuropeptide expression and morphometric differences in crushed alveolar inferior nerve of rats: effects of photobiomodulation. Lasers Med Sci 32:833–840

  34. 34.

    Souza-Barros L, Dhaidan G, Maunula M et al (2018) Skin color and tissue thickness effects on transmittance, reflectance, and skin temperature when using 635 and 808 nm lasers in low intensity therapeutics. Lasers Surg Med 50:291–301

  35. 35.

    Nasouri B, Murphy TE, Berberoglu H (2014) Simulation of laser propagation through a three-layer human skin model in the spectral range from 1000 to 1900 nm. J Biomed Opt 19:075003

  36. 36.

    Miloro M, Criddle TR (2018) Does low-level laser therapy affect recovery of lingual and inferior alveolar nerve injuries? J Oral Maxillofac Surg 76:2669–2675

  37. 37.

    Miloro M, Halkias LE, Mallery S et al (2002) Low-level laser effect on neural regeneration in Gore-Tex tubes. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 93:27–34

  38. 38.

    Angius D, Wang H, Spinner RJ et al (2012) A systematic review of animal models used to study nerve regeneration in tissue-engineered scaffolds. Biomaterials 33:8034–8039

  39. 39.

    Wang X, Reddy DD, Nalawade SS et al (2017) Impact of heat on metabolic and hemodynamic changes in transcranial infrared laser stimulation measured by broadband near-infrared spectroscopy. Neurophotonics 5(1):011004

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Funding

This research was funded by Baskent University Research Fund (Grand Number D-DA 14/06).

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Correspondence to Nurettin Diker.

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The authors declare that they have no conflict of interest.

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All procedures performed in the present study, involving animals, were approved by the Institutional Review Board and Ethical Committee of Baskent University for experimental research on animals.

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Diker, N., Aytac, D., Helvacioglu, F. et al. Comparative effects of photobiomodulation therapy at wavelengths of 660 and 808 nm on regeneration of inferior alveolar nerve in rats following crush injury. Lasers Med Sci 35, 413–420 (2020). https://doi.org/10.1007/s10103-019-02838-w

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Keywords

  • Photobiomodulation
  • Wavelength
  • Inferior alveolar nerve
  • Nerve injury
  • Biostimulation