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The study of killing effect and inducing apoptosis of 630-nm laser on lung adenocarcinoma A549 cells mediated by hematoporphyrin derivatives in vitro

  • Cunzhi Lin
  • Yuanyuan Zhang
  • Xinhong Zhu
  • Shichao Cui
  • Yiwei Cao
  • Rong Li
  • Lijun WangEmail author
Original Article

Abstract

To investigate the killing effect and inducing apoptosis of 630-nm laser mediated by hematoporphyrin derivatives (HPD) on human lung adenocarcinoma A549 cells. The human lung adenocarcinoma A549 cells were incubated at random with different concentrations of HPD (5, 10, 12, 15, 20 μg/ml) for 4 h and then illuminated by 630-nm laser with different energy densities (25, 50, 75, 100 mW/cm2). And, meanwhile, the simple photosensitizer group, laser irradiation group, and blank control group were established. Then, CCK8, Hoechst 33258 staining, RT-PCR, and Western blot were employed. HPD-PDT proved no killing effect on the lung adenocarcinoma A549 cells with photosensitizer or laser irradiation alone. With the combination, the killing effect was obvious. CCK8 showed that the A549 cell viability in 15 μg/ml and 20 μg/ml HPD group as well as 50 mW/cm2, 75 mW/cm2, and 100 mW/cm2 power density group decreased significantly compared with the control group. Hoechst 33258 staining showed that with the increase of HPD concentration, the cells presented chromatin fixation and hyperchromatic nuclei. The Annexin V-FITC/PI double staining was used to detect the apoptosis rate, and the difference was statistically significant. RT-PCR and Western blot showed that the expression of Caspase-3 and Bax were significantly up-regulated. However, the Bcl-2 and Survivin were significantly down-regulated in the HPD-PDT group, while those of the other three groups showed no significant changes. HPD-PDT has a significant effect on A549 cells. The mechanism of action may be related to the upregulation of the expression of Caspase-3, Bax, and downregulation of the expression of Bcl-2 and Survivin.

Keywords

Photodynamic therapy Hematoporphyrin derivative A549 cells Apoptosis Gene expression 

Notes

Funding information

This work was supported by the grants from Huangdao Science and Technology Bureau Project of Qingdao (2014-1-77).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Cunzhi Lin
    • 1
  • Yuanyuan Zhang
    • 1
  • Xinhong Zhu
    • 2
  • Shichao Cui
    • 1
  • Yiwei Cao
    • 1
  • Rong Li
    • 1
  • Lijun Wang
    • 2
    Email author
  1. 1.Department of RespirationThe Affiliated Hospital of Qingdao UniversityQingdaoChina
  2. 2.Department of Internal MedicineQingdao Municipal HospitalQingdaoChina

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