Topical and intradermal delivery of PpIX precursors for photodynamic therapy with intense pulsed light on porcine skin model
- 149 Downloads
In order to purposely decrease the time of the photodynamic therapy (PDT) sessions, this study evaluated the effects of PDT using topical and intradermal delivery of two protoporphyrin (PpIX) precursors with intense pulsed light (IPL) as irradiation source. This study was performed on porcine skin model, using an IPL commercial device (Intense Pulse Light, HKS801). IPL effect on different administration methods of two PpIX precursors (ALA and MAL) was investigated: a topical cream application and an intradermal application using a needle-free, high-pressure injection system. Fluorescence investigation showed that PpIX distribution by needle-free injection was more homogeneous than that by cream, suggesting that a shorter drug-light interval in PDT protocols is possible. The damage induced by IPL-PDT assessed by histological analysis mostly shows modifications in collagens fibers and inflammation signals, both expected for PDT. This study suggested an alternative protocol for the PDT treatment, possibility half of the incubation time and with just 3 min of irradiation, making the IPL-PDT, even more, promising for the clinical treatment.
KeywordsPhotodynamic therapy Intense pulsed light Porcine skin Aminolevulinic acid Methyl aminolevulinate
We acknowledge the support from the Center for Research in Optics and Photonics (CEPOF - FAPESP CePID program grant 2013/07276-1).
This study was funded in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 and by grants 2013/07276-1 (CePOF), 2009/54035-4 (EMU) of São Paulo Research Foundation (FAPESP).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.
- 9.Oh SH, Ryu DJ, Han EC et al (2009) A comparative study of topical 5-aminolevulinic acid incubation times in photodynamic therapy with intense pulsed light for the treatment of inflammatory acne. Dermatol Surg 35:1918–1926. https://doi.org/10.1111/j.1524-4725.2009.01315.x CrossRefPubMedGoogle Scholar
- 10.Cecília M, Costa D, Keiko A et al (2017) Photodynamic Therapy with 5- Aminolevulinic Acid (ALA) in the Treatment of Acne: A Case Study. Clin Dermatol Res Ther. 2017; 1(1):114Google Scholar
- 13.Rhodes LE, de Rie M, Enström Y et al (2004) Photodynamic therapy using topical methyl aminolevulinate vs surgeryfor nodular basal cell carcinoma. Arch Dermatol 140. https://doi.org/10.1001/archderm.140.1.17
- 14.Morton CAA, Szeimies R-MM, Sidoroff A, Braathen LRR (2013) European guidelines for topical photodynamic therapy part 1: treatment delivery and current indications - actinic keratoses, Bowen’s disease, basal cell carcinoma. J Eur Acad Dermatol Venereol 27:536–544. https://doi.org/10.1111/jdv.12031 CrossRefPubMedGoogle Scholar
- 17.Santos MAV, Belo VG, Santos G (2005) Effectiveness of photodynamic therapy with topical 5-aminolevulinic acid and intense pulsed light versus intense pulsed light alone in the treatment of acne vulgaris: comparative study. Dermatol Surg 31:910–915. https://doi.org/10.1111/j.1524-4725.2005.31804 CrossRefPubMedGoogle Scholar
- 19.Shaaban D, Abdel-Samad Z, El-Khalawany M (2012) Photodynamic therapy with intralesional 5-aminolevulinic acid and intense pulsed light versus intense pulsed light alone in the treatment of acne vulgaris: a comparative study. Dermatol Ther 25:86–91. https://doi.org/10.1111/j.1529-8019.2012.01427.x CrossRefPubMedGoogle Scholar
- 22.Gracielli Sousa RP, de Menezes PFC, Fujita AKL et al (2014) Microneedles rollers as a potential device to increase ALA diffusion and PpIX production: evaluations by wide-field fluorescence imaging and fluorescence spectroscopy. Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 892614. https://doi.org/10.1117/12.2040618
- 27.Liu J, Kim D, Brown LD et al (2009) Comparison of human, porcine, and rodent wound healing with new miniature swine study data. Journal of the American Association for Laboratory Animal Science: JAALAS.48. 581-581Google Scholar
- 32.Blanco KC, Moriyama LT, Inada NM et al (2015) Fluorescence guided PDT for optimization of the outcome of skin cancer treatment. Front Phys 3. https://doi.org/10.3389/fphy.2015.00030
- 34.Grecco C, Buzzá HH, Stringasci MD et al (2015) Single LED-based device to perform widefield fluorescence imaging and photodynamic therapy. Proc. SPIE 9531, Biophotonics South America, 953121. https://doi.org/10.1117/12.2185925
- 38.Wan MT, Lin JY. (2014) Current evidence and applications of photodynamic therapy in dermatology. Clinical, Cosmetic and Investigational Dermatology. https://doi.org/10.2147/CCID.S35334
- 39.Requena MB, Stringasci MD, Pratavieira S et al (2018) Thermographic analysis of photodynamic therapy with intense pulsed light and needle-free injection photosensitizer delivery: an animal study. Proc. SPIE 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis, 104891F. https://doi.org/10.1117/12.2300407
- 40.Friedmann DP, Goldman MP, Fabi SG et al (2014) The effect of multiple sequential light sources to activate Aminolevulinic acid in the treatment of actinic Keratoses: a retrospective study. J Clin AestheticDermatol. 7:20–25Google Scholar