Irradiation by blue light-emitting diode enhances osteogenic differentiation in gingival mesenchymal stem cells in vitro
- 122 Downloads
The aim of this study was to investigate the effects of blue light irradiation on the process of osteogenic differentiation in stem cells. The cells used in this study were derived from human gingival mesenchymal stem cells (hGMSCs), and were treated with 0 (control group), 1, 2, 4 or 6 J/cm2 blue light using blue light-emitting diodes. Cell growth was assessed by the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-Diphenyl-2H-tetrazolium bromide (MTT) cell proliferation assay and osteogenic differentiation was evaluated by monitoring alkaline phosphatase (ALP) activity, alizarin red staining and real-time PCR (RT-PCR). The results of the MTT assay indicated that blue light inhibited hGMSC proliferation, and the ALP and alizarin red results showed that blue light promoted osteogenesis. The expression levels of the osteogenic genes runt-related transcription factor2 (Runx2), collagen type I (Col1) and osteocalcin (OCN) increased significantly (P < 0.05) when cells were irradiated with 2 or 4 J/cm2 of blue light. In conclusion, irradiation with blue light inhibits the proliferation of hGMSC and promotes osteogenic differentiation.
KeywordsBlue light-emitting diode Osteogenic differentiation Mesenchymal stem cells (MSCs) Gingiva
This work was supported by the Luzhou Municipal People’s Government-Southwest Medical University science and technology strategic cooperation projects of China (no. 2017LZXNYD-T03), Luzhou Municipal Science and Technology Bureau of China (no. 2016-R-70(13/24)). The reagents of this study were supported by these funds that all came from Southwest Medical University.
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no conflict of interest.
All procedures performed in the study were in accordance with the Ethics Committee of the Affiliated Hospital of Stomatology Southwest Medical University Certificate (contract grant 20180314001) and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.
- 5.Tomar GB, Srivastava RK, Gupta N, Barhanpurkar AP, Pote ST, Jhaveri HM, Mishra GC, Wani MR (2010) Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine. Biochem Biophys Res Commun 393(3):377CrossRefGoogle Scholar
- 14.Amid R, Kadkhodazadeh M, Ahsaie MG, Hakakzadeh A (2014) Effect of low level laser therapy on proliferation and differentiation of the cells contributing in bone regeneration. J Lasers Med Sci 5(4):163–170Google Scholar
- 25.Li WT, Chen CW, Huang PY (2013) Effects of low level light irradiation on the migration of mesenchymal stem cells derived from rat bone marrow. Conf Proc IEEE Eng Med Biol Soc 2013:4121–4124Google Scholar
- 29.Mikami R, Mizutani K, Aoki A, Tamura Y, Aoki K, Izumi Y (2017) Low-level ultrahigh-frequency and ultrashort-pulse blue laser irradiation enhances osteoblast extracellular calcification by upregulating proliferation and differentiation via transient receptor potential vanilloid 1. Lasers Surg Med 50(4):340–352CrossRefGoogle Scholar
- 33.Liu TCY, Duan R, Yin PJ, Li Y, Li SL (2000) Membrane mechanism of low-intensity laser biostimulation on a cell. In: Biomedical Photonics and Optoelectronic Imaging pp 186–192Google Scholar
- 34.Liu TCY, Jiao JL, Xu XY, Liu XG, Deng SX, Liu SH (2005) Photobiomodulation: phenomenology and its mechanism. Proceedings of SPIE. The Int Soc Opt Eng 5630:185–191Google Scholar
- 35.Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS (1990) Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143(3):420–430CrossRefGoogle Scholar
- 37.Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, Sato M, Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T (1997) Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89(5):755–764CrossRefGoogle Scholar