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Photobiomodulation therapy modulates epigenetic events and NF-κB expression in oral epithelial wound healing

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Abstract

The aim of this study was to evaluate the effect of photobiomodulation therapy (PBMT) on histone 3 acetylation (acH3) and NF-κB expression during oral ulcer healing. A total of 48 male Wistar rats were divided into control group (CG) and PBMT group (n = 24 each). Traumatic ulcers were created in the dorsum of the rats’ tongue with a punch tool. Irradiation with InGaAlP laser, 660 nm, 40 mW, 0.04 cm2 spot size, 4 J/cm2, 4 s, and 0.16 J per spot was performed once a day in close contact for 10 consecutive days. CG received only daily handling. Rats were euthanized on days 3, 5, and 10 (n = 8) and were monitored daily to assess wound status. Immunohistochemical analysis for acH3 and NF-κB detection was performed. One thousand epithelial cells were counted, and mean acH3- and NF-κB-positive cells were calculated and compared between the groups. PBMT accelerated the repair of oral ulcers. On day 3, PBMT showed significantly higher means for acH3- and NF-κB-positive cells than CG. On day 5, no difference was observed between the groups concerning both markers. On day 10, PBMT presented lower acH3 and NF-κB means than the control group. We concluded that PBMT stimulates keratinocyte migration in the early stage of oral wound healing and keratinocyte differentiation at the final stage by modulating histone acetylation and NF-κB expression.

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Acknowledgments

The authors are grateful to Marta Justina Giotti Cioato and Flavia Rejane Giusti for technical support.

Funding

This study was funded by the Postgraduate Research Group of Porto Alegre Clinics Hospital (GPPG/FIPE: 2014-0534), Brazilian National Council for Scientific and Technological Development (CNPq student scholarship), Children’s Cancer Institute (student scholarship), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil(CAPES)-finance code 001. Manoela Domingues Martins is a research fellow funded by the Brazilian National Council for Scientific and Technological Development (CNPq).

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Authors and Affiliations

  1. Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

    Amanda de Farias Gabriel, Vivian Petersen Wagner, Cintia Correa, Liana Preto Webber, Vinicius Coelho Carrard & Manoela Domingues Martins

  2. Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil

    Vivian Petersen Wagner & Manoela Domingues Martins

  3. Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA

    Liana Preto Webber

  4. Experimental Pathology Unit, Clinics Hospital of Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

    Emily Ferreira Salles Pilar & Manoela Domingues Martins

  5. School of Dentistry, University of Caxias do Sul, Caxias do Sul, RS, Brazil

    Marina Curra

  6. Department of Oral Medicine, Porto Alegre Clinics Hospital (HCPA/UFRGS), Porto Alegre, RS, Brazil

    Vinicius Coelho Carrard, Marco Antonio Trevizani Martins & Manoela Domingues Martins

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  1. Amanda de Farias Gabriel
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  2. Vivian Petersen Wagner
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  6. Marina Curra
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  7. Vinicius Coelho Carrard
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  9. Manoela Domingues Martins
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Corresponding author

Correspondence to Manoela Domingues Martins.

Ethics declarations

The study was approved by the ethics committee of Porto Alegre Clinics Hospital (Brazil) under process number 14-0534. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed involving animals were in accordance with the ethical standards of the institution, which the study was conducted.

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de Farias Gabriel, A., Wagner, V.P., Correa, C. et al. Photobiomodulation therapy modulates epigenetic events and NF-κB expression in oral epithelial wound healing. Lasers Med Sci 34, 1465–1472 (2019). https://doi.org/10.1007/s10103-019-02745-0

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