To evaluate the feasibility of a 980-nm contact diode laser (CDL) as a method for creating a posterior laryngofissure in live pigs. Twenty-eight Landrace pigs (15–20 kg) were anesthetized, intubated, ventilated, and submitted to a cervical tracheostomy. An anterior and posterior midline longitudinal laryngofissure incision was created according to randomization—control (n = 4), posterior laryngofissure with a scalpel blade; electrocautery (n = 12), posterior laryngofissure by electrocautery (10, 15, 20, 25 W powers); CDL (n = 12), posterior laryngofissure by the CDL (10, 15, 20, 25 W peak powers in pulsed mode). Larynx and proximal trachea were excised, prepared for histopathology, and digital morphometric analysis. Measurements in and within each group were analyzed (Kruskal-Wallis and Dunn test) with a level of significance of p < 0.05. Incision width was not different between the groups, as well as in the powers used in CDL (p = 0.161) and electrocautery group (p = 0.319). The depth of the incisions was smaller in the Laser group compared to control (p = 0.007), and in the electrocautery compared to control (p = 0.026). Incision area was smaller in CDL compared with the control (p = 0.027), and not different between laser and electrocautery groups (p = 0.199). The lateral thermal damage produced by electrocautery was the largest, with a significant difference between laser and electrocautery (p = 0.018), and between electrocautery and control (p = 0.004), whereas the comparison between laser and control showed no significant differences (p = 0.588). The posterior laryngofissure incision using a 980-nm CDL is feasible resulting in smaller incisional area and less lateral thermal damage.
Contact diode laser Larynx Pigs Tracheal stenosis Surgery
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Role of funding source
This study was funded by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil (FAPESP grants 2015/17847-1 and 2016/25437-0). Funding covered the experimental expenses, the equipment used in the study, and a scholarship for a medical student.
Compliance with ethical standards
Conflict of interest statement
The author Marilia Wellichan Mancini is a physicist working at the company DMC at the department of research and development of the laser equipment used in this study. The author Paulo Francisco Guerreiro Cardoso was the recipient of the FAPESP grants 2015/17847-1 and 2016/25437-0. The remaining authors have no conflict of interest to disclose.
The research protocol was approved on by the ethics committee (CEUA 153/14) of the Faculty of Medicine of the University of Sao Paulo, Brazil.
Not applicable in the current animal experimental protocol.
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