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Q-switched 1064-nm dymium-doped yttrium aluminum garnet laser irradiation induces skin collagen synthesis by stimulating MAPKs pathway

  • Zhi Yang
  • Huiyi Xiang
  • Xiaoxia Duan
  • Jianmeng Liu
  • Xiaolin He
  • Yunting He
  • Shaoyu Hu
  • Li HeEmail author
Original Article
  • 43 Downloads

Abstract

The 1064-nm Q-switched neodymium-doped yttrium aluminum garnet (Nd:YAG) laser is widely used in clinical practice. However, the effects of 1064-nm Q-switched Nd:YAG laser on skin collagen generation have not been fully elucidated. The objectives of the present study were to investigate whether the 1064-nm Q-switched Nd:YAG laser can be used for non-ablative rejuvenation and to explore the possible mechanism underlying the effects. Six-week-old SKH-1 hairless mice were irradiated by the 1064-nm Nd:YAG laser at fluences of 0, 0.5, 1, 1.5, and 2 J/cm2, respectively. The contents of hydroxyproline and hydration were detected after laser irradiation. Moreover, hematoxylin-eosin (HE) staining was preformed to evaluate the dermal thickness. Immunofluorescence was used to detect the expressions of MMP-2 and TIMP-1 in the skin after laser irradiation. Furthermore, qRT-PCR was performed to determine the expressions of TGF-β1 and Smad3. In addition, the expressions of ERK1/2, p-ERK1/2, p38, p-p38, JNK, ERK5, and collagen were evaluated by Western blotting. The results indicated that the levels of hydroxyproline, hydration, and collagen were markedly increased; both the thickness of dermal was enhanced after low dose of laser treatment. Moreover, the expression of TIMP-1 was significantly increased, whereas the expression of MMP-2 was remarkably decreased after laser irradiation. Meanwhile, TGF-β1, Smad3, p-ERK1/2, p-P38, and JNK productions were significantly enhanced in irradiated group compared with the ones non-irradiated. Nevertheless, no significant changes were observed in the expression of ERK5 after irradiation. In summary, our study demonstrated that Q-switched 1064-nm Nd:YAG laser can induce collagen generation, at least in part, through activating TGF-β1/Smad3/MAPK signaling pathway.

Keywords

1064-nm Q-switched neodymium-doped yttrium aluminum garnet laser Collagen MAPKs ERK1/2 p38MAPK 

Notes

Funding information

This work was performed in Department of Dermatology, the first affiliated hospital of Kunming medical university and supported by the National Natural Science Foundation of China (81560507) and the Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Research Fund for Applied Basic Research (2014FB023).

Compliance with ethical standards

Conflict of interest

This authors declare that they have no conflict of interest.

Ethical approval

This study received ethical approval from the first affiliated hospital of Kunming medical university ethics committee (approval no. 2017-06).

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of DermatologyThe first affiliated hospital of Kunming medical UniversityKunmingChina

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