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Lasers in Medical Science

, Volume 34, Issue 3, pp 525–535 | Cite as

Biochemical changes in injured sciatic nerve of rats after low-level laser therapy (660 nm and 808 nm) evaluated by Raman spectroscopy

  • Melissa de Almeida Melo Maciel Mangueira
  • Nilton Maciel MangueiraEmail author
  • Ozimo Pereira Gama Filho
  • Márcio Moysés de Oliveira
  • Renato Albuquerque Heluy
  • Landulfo SilveiraJr
  • Egas Caparelli Moniz de Aragão Dáquer
Original Article

Abstract

The aim of this study was to identify biochemical changes in sciatic nerve (SN) after crush injury and low-level laser therapy (LLLT) with 660 nm and 808 nm by Raman spectroscopy (RS) analysis. A number of 32 Wistar rats were used, divided into four groups (control 1, control 2, LASER 660 nm, and LASER 808 nm). All animals underwent surgical procedure of the SN and groups control 2, LASER 660 nm, and LASER 808 nm were submitted to SN crush damage (axonotmesis). The LLLT in the groups LASER 660 nm and LASER 808 nm was applied daily for 21 consecutive days (100 mW, 30 s, 133 J/cm2 fluence). The hind paw was removed and the SN was dissected and positioned on an aluminum support to collect dispersive Raman spectra (830 nm excitation, 30 s accumulation). To estimate the biochemical changes in the SN associated with LLLT, the principal component analysis (PCA) was applied. The Raman spectra of the sciatic nerve fragments showed peaks of the major biochemical components of the nerve, especially sphingolipids, phospholipids, glycoproteins, and collagen. The spectral features identified in some of the principal component loading vectors are referred to the biochemical elements present on the SN and were increased in the groups treated with LLLT, mainly lipids (sphingo and phospholipids) and proteins (collagen)—constituents of the myelin sheath. The RS was effective in identifying the biochemical differences in the SN after the crush injury, and LASER 660 nm was more efficient than the LASER 808 nm in cell proliferation and repair of the injured SN.

Keywords

Peripheral nerve Low-level laser therapy Raman spectroscopy Biochemical changes 

Notes

Funding information

The authors thank FAPEMA (Foundation for Research Support and Scientific and Technological Development of the Maranhão) for the research grant support (Universal Process no. 01377/16), FAPESP (São Paulo Research Foundation) for the partial financial support (Grant no. 2009/01788-5), and CNPq (National Council for Scientific and Technological Development) for the productivity fellowship (Process no. 306344/2017-3). The authors also thank LACEMA (Academic League of Experimental Surgery of Maranhão).

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical approval

It is approved by Commission of Ethics in the Use of Animals (CEUA) from Federal University of Maranhão (protocol no. 23115.005396/2016-65).

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  • Melissa de Almeida Melo Maciel Mangueira
    • 1
  • Nilton Maciel Mangueira
    • 2
    Email author
  • Ozimo Pereira Gama Filho
    • 2
  • Márcio Moysés de Oliveira
    • 2
  • Renato Albuquerque Heluy
    • 2
  • Landulfo SilveiraJr
    • 3
  • Egas Caparelli Moniz de Aragão Dáquer
    • 4
  1. 1.Department of PhysiotherapyFaculdade Santa Terezinha – CESTSão LuísBrazil
  2. 2.Department of MorphologyUniversidade Federal do Maranhão – UFMASão LuísBrazil
  3. 3.Center for Innovation, Technology and Education – CITEUniversidade Anhembi Morumbi – UAMSão José dos CamposBrazil
  4. 4.Physiological Sciences Department, Roberto Alcântara Gomes Biology InstituteRio de Janeiro State UniversityRio de JaneiroBrazil

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