Application of continuous-wave photoacoustic sensing to red blood cell morphology
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The feasibility of continuous wave laser-based photoacoustic (CWPA) response technique in detecting the morphological changes in cells during the biological studies, through the features extracted from CWPA signal (i.e., amplitude) is demonstrated here. Various hematological disorders (e.g., sickle cell anemia, thalesemia) produce distinct changes at the cellular level morphologically. In order to explore the photoacoustic response technique to detect these morphological changes, we have applied CWPA technique onto the blood samples. Results of our preliminary study show a distinct change in the signal amplitude of photoacoustic (PA) signal due to a change in the concentration of blood, which signifies the sensitivity of the technique towards red blood cell (RBC) count (related to hematological disease like anemia). Further hypotonic and hypertonic solutions were induced in blood to produce morphological changes in RBCs (i.e., swollen and shrink, respectively) as compared to the normal RBCs. Experiments were performed using continuous wave laser-based photoacoustic response technique to verify the morphological changes in these RBCs. A distinct change in the PA signal amplitude was found for the distinct nature of RBCs (swollen, shrink, and normal). Thus, this can serve as a diagnostic signature for different biological studies based on morphological changes at cellular level. The experiments were also performed using conventional pulsed laser photoacoustic response technique which uses nano-second pulsed laser and the results obtained from both PA techniques were validated to produce identical changes. This demonstrates the utility of continuous wave laser-based photoacoustic technique for different biological studies related to morphological cellular disorders.
KeywordsContinuous wave laser based photoacoustic (CWPA) Red blood cell Morphological cellular disorder PA amplitude
This work is financially supported by Department of Biotechnology, India (Grant No. BT/PR/5879/MED/32/241/2012).
Compliance with ethical standards
Conflict of interest
The authors declares that they have no conflict of interest.
Since in this study, human blood from volunteer healthy donors was used as a standard sample (ethical clearance was obtained from bio-safety and bio-ethics committee, Indian Institute of Technology Indore) to perform experiments.
- 1.Xu M, Wang LV (2006) Photoacoustic imaging in biomedicine. Rev Sci Instrum 77:–041101, 22Google Scholar
- 3.Beard P (2011) Biomedical photoacoustic imaging. Interface Focus 1:602–631. https://doi.org/10.1098/rsfs.2011.0028
- 4.Wang X, Chamberland DL, Jamadar DA (2007) Noninvasive photoacoustic tomography of human peripheral joints toward diagnosis of inflammatory arthritis. Optics Letters 32(20):3002–3004Google Scholar
- 10.Marion A et al (2011) A quantitative study to design an experimental setup for photoacoustic imaging. In: Engineering in Medicine and Biology Society. EMBC, 2011 Annual International Conference of the IEEE, pp 7211–7214Google Scholar
- 12.An R et al (2014) Spatially variant red blood cell crenation in altering current non-uniform. Biomicrofluidics 8:421–425Google Scholar
- 15.Prahl S (1999) Optical absorption of haemoglobin. Tech Rep, Oregon Medical Laser Centre, Portland, OregonGoogle Scholar
- 19.Glader BE et al (1978) Cation permeability alterations during sickling: relationship to cation composition and cellular hydration of irreversibly sickled cells. Blood 51:983–989Google Scholar
- 20.Hebble RP (1991) Beyond Haemoglobin Polymerization: the red blood cell membrane and sickle disease pathophysiology. Blood 77:214–237Google Scholar