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Photobiomodulation of the microbiome: implications for metabolic and inflammatory diseases

  • Brian Bicknell
  • Ann Liebert
  • Daniel Johnstone
  • Hosen Kiat
Original Article

Abstract

The human microbiome is intimately associated with human health, with a role in obesity, metabolic diseases such as type 2 diabetes, and divergent diseases such as cardiovascular and neurodegenerative diseases. The microbiome can be changed by diet, probiotics, and faecal transplants, which has flow-on effects to health outcomes. Photobiomodulation has a therapeutic effect on inflammation and neurological disorders (amongst others) and has been reported to influence metabolic disorders and obesity. The aim of this study was to examine the possibility that PBM could influence the microbiome of mice. Mice had their abdomen irradiated with red (660 nm) or infrared (808 nm) low-level laser, either as single or multiple doses, over a 2-week period. Genomic DNA extracted from faecal pellets was pyrosequenced for the 16S rRNA gene. There was a significant (p < 0.05) difference in microbial diversity between PBM- and sham-treated mice. One genus of bacterium (Allobaculum) significantly increased (p < 0.001) after infrared (but not red light) PBM by day 14. Despite being a preliminary trial with small experimental numbers, we have demonstrated for the first time that PBM can alter microbiome diversity in healthy mice and increase numbers of Allobaculum, a bacterium associated with a healthy microbiome. This change is most probably a result of PBMt affecting the host, which in turn influenced the microbiome. If this is confirmed in humans, the possibility exists for PBMt to be used as an adjunct therapy in treatment of obesity and other lifestyle-related disorders, as well as cardiovascular and neurodegenerative diseases. The clinical implications of altering the microbiome using PBM warrants further investigation.

Keywords

Photobiomodulation Microbiome Allobaculum Infrared laser 

Notes

Acknowledgements

The authors would like to thank Elvis Freeman-Acquah who assisted with the PBM treatments and collection of faeces and Lyudmyla Arshynnikova who translated manuscripts in the Russian language.

Author contribution

BB, AL, and DJ—design and implementation the study; BB and DJ—acquisition of data; BB—analysis and interpretation; BB, AL, and HK—drafting of manuscript; all authors—revision and approval.

Funding

DJ was supported by the Early Career Fellowship from the National Health and Medical Research Council (NHMRC) of Australia.

Compliance with ethical standards

Conflict of interest

BB is an agent for Spectro Analytic Irradia AB, the company that manufactures the Irradia laser products supplied for this experiment. The other authors declare that they have no conflict of interest.

Ethics approval

All experiments were approved by the Animal Ethics Committee of University of Sydney (Protocol Number: 2017/1128).

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Australasian Research InstituteWahroongaAustralia
  2. 2.Faculty of Health SciencesAustralian Catholic UniversityNorth SydneyAustralia
  3. 3.Department of MedicineUniversity of SydneyCamperdownAustralia
  4. 4.Bosch InstituteUniversity of SydneyCamperdownAustralia
  5. 5.Faculty of Medicine and Health SciencesMacquarie UniversityWest RydeAustralia
  6. 6.School of Medical SciencesUniversity of New South WalesKensingtonAustralia

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