Lasers in Medical Science

, Volume 33, Issue 9, pp 1961–1968 | Cite as

Evaluation of the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit ears

  • Wen Zhang
  • Xingjian Cheng
  • Huizheng Li
  • Mo CaoEmail author
Original Article


To evaluate the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit ears to provide an experimental basis and theoretical foundation for the treatment of hypertrophic scars. Hypertrophic scars were established on the ventral surface of the ears of six New Zealand white rabbits. Left and right ears were randomly divided into two groups: experimental group treated with micro-plasma radio frequency and control group with no treatment. H&E staining and CD34 labeling of microvessels were performed to analyze ear specimens, and immunohistochemical staining was conducted to detect IL-8 and MCP-1 in the scars. Compared with the control group, scar tissue in the experimental group was improved by color and texture. H&E-stained collagen fiber bundles were more organized after treatment as assessed by optical microscopy. The number of microvessels in the experimental group was decreased compared with that in the control group. Microvascular density was significantly reduced in the experimental group compared with the control group (27.16 ± 5.64 and 48.75 ± 8.25 mm2, respectively; P < 0.01). The mean optical densities of IL-8 and MCP-1 were significantly reduced in the experimental group compared with the control group (IL-8 0.016 ± 0.011 and 0.078 ± 0.023, respectively; MCP-1 0.018 ± 0.016 and 0.054 ± 0.038, respectively; both P < 0.01). The micro-plasma radio-frequency technique has a therapeutic effect on hypertrophic scars in rabbit ears.


Micro-plasma radio-frequency technique Hypertrophic scar Microvascular IL-8 MCP-1 


Compliance with ethical standards

Conflict of interest statement

The authors declare that they have no conflict of interest.

Ethical approval

The present study was approved by our institutional review board.


  1. 1.
    Halachmi S, Orenstein A, Meneghel T et al (2010) A novel fractional micro plasma radio frequency technology for the treatment of facial scars and rhytids: a pilot study. J Cosmet Laser Ther 12(5):208–212CrossRefGoogle Scholar
  2. 2.
    Kilmer S, Semchyshyn N, Shah G et al (2007) A pilot study on the use of a plasma skin regeneration device (Portrait PSR3) in full facial r-ejuvenation procedures[J]. Lasers Med Sci 22(2):101–109CrossRefGoogle Scholar
  3. 3.
    Li H, Liu J, Xia W et al (2001) Establishment and application of experimental animal model for hypertrophic scar. Chin J Plast Surg 17(5):276–278Google Scholar
  4. 4.
    Finnerty CC, Jeschke MG, Branski LK, et al (2016) Hypertrophic scarring: the greatest unmet challenge after burn injury. Lancet 388(10052):1427–1436CrossRefGoogle Scholar
  5. 5.
    Bae YC, Alabdulrazzaq H, Brauer JA et al (2017) Treatment of recalcitrant port-wine stains (PWS) using a combined pulsed dye laser (PDL) and radiofrequency (RF) energy device. J Am Acad Dermatol 76(2):321–326CrossRefGoogle Scholar
  6. 6.
    Choi JE, Oh GN, Kim JY et al (2014) Ablative fractional laser treatment for hypertrophic scars: comparison between Er:YAG and CO2 fractional lasers. J Dermatolog Treat 25(4):299–303CrossRefGoogle Scholar
  7. 7.
    Wang S, Mi J, Li Q et al (2017) Fractional micro plasma radiofrequency technology for non-hypertrophic post-burn scars in Asians: a prospective study of 95 patients. Lasers Surg Med 49(6):563–569CrossRefGoogle Scholar
  8. 8.
    Gold MH, Berman B, Clementoni MT et al (2014) Updated international clinically commendations on scar management: part 1: evaluating the evidence. Dermatol Surg 40:817–824PubMedGoogle Scholar
  9. 9.
    Lan T, Yin R (2017) Research progress of micro-plasma radio-frequency technology in the treatment of scar. Chin J Aesthetic Med 26(3):125–128Google Scholar
  10. 10.
    A Li, Dubey S, Varney ML et al (2003) IL-8 directly enhanced endothelial cell survival, proliferation, and matrix metalloproteinases production and regulated angiogenesis. J Immunol 170(6):3369–3376CrossRefGoogle Scholar
  11. 11.
    Schraufstatter IU, Chung J, Burger M (2001) IL-8 activates endothelial cell CXCR1 and CXCR2 the rough Rho and Rac signaling pathways. Am J Physiol Lung Cell Mol Physiol 280:1094–1103CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  • Wen Zhang
    • 1
  • Xingjian Cheng
    • 1
  • Huizheng Li
    • 1
  • Mo Cao
    • 1
    Email author
  1. 1.Department of Plastic Surgerythe Third Hospital of Hebei Medical UniversityShijiazhuangPeople’s Republic of China

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