Lasers in Medical Science

, Volume 33, Issue 8, pp 1723–1731 | Cite as

Bactericide effect of methylene blue associated with low-level laser therapy in Escherichia coli bacteria isolated from pressure ulcers

  • Thais Ferreira Gomes
  • Matheus Masalskiene Pedrosa
  • Ana Claudia Laforga de Toledo
  • Veridiana Wanshi Arnoni
  • Mirian dos Santos Monteiro
  • Davi Cury Piai
  • Silvia Helena Zacarias Sylvestre
  • Bruno FerreiraEmail author
Original Article


The present study analyzed the bactericidal effect of methylene blue associated with low-level lasers on Escherichia coli isolated from a pressure ulcer. Microbiological material from a pressure ulcer was isolated using an aseptic swab, and antimicrobial activity was verified using the diffusion disc method. Methylene blue was used at concentrations of 0.001 and 0.005%, and low-level lasers of 670, 830, and 904 nm, with the energy densities of 4, 8, 10, and 14 J/cm2, were tested on three plates each and combined with methylene blue of each concentration. In addition, three control plates were used, with each concentration and energy density separated without any interventions. The results were analyzed using the paired sample t test to determine the bactericidal effect of the methylene blue and using the ANOVA test to compare the effects of the energy densities and wavelengths among the low-level laser treatment protocols. The results showed bacterial reduction at wavelengths of 830 and 904 nm and more proliferation in wavelengths of 670 nm. In wavelength of 830 nm, a bacterial reduction was observed in the conditions with 0.001% methylene blue in all energy density utilized, with 0.005% methylene blue in energy density of 10 J/cm2, and without methylene blue in energy density at 10 J/cm2. And in a wavelength of 904 nm, all condition showed bacterial reduction with or without methylene blue. We concluded that the low-level lasers of 904 and 830 nm have bactericidal effects and at better energy densities (10 and 14 J/cm2).


Methylene blue Low-level laser Pressure ulcer 



We thank the Department of Physical Therapy and Study Center and Research on Regional Development (CEPed) of the University Center UNIFAFIBE, SP.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This research was approved by the Research Ethics Committee (number CAAE: 67741017.4.0000.5387).


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  • Thais Ferreira Gomes
    • 1
  • Matheus Masalskiene Pedrosa
    • 1
  • Ana Claudia Laforga de Toledo
    • 1
  • Veridiana Wanshi Arnoni
    • 2
  • Mirian dos Santos Monteiro
    • 1
  • Davi Cury Piai
    • 1
  • Silvia Helena Zacarias Sylvestre
    • 1
  • Bruno Ferreira
    • 1
    Email author
  1. 1.Department of Physical therapyUniversity Center UNIFAFIBEBebedouroBrazil
  2. 2.Study Center CollucciCollucci’s ClinicalRibeirão PretoBrazil

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