Lasers in Medical Science

, Volume 33, Issue 7, pp 1455–1460 | Cite as

Time-dependent antimicrobial effect of photodynamic therapy with TONS 504 on Pseudomonas aeruginosa

  • Kentaro Sueoka
  • Taiichiro ChikamaEmail author
  • Miftahul Akhyar Latief
  • Ji-Ae Ko
  • Yoshiaki Kiuchi
  • Takemasa Sakaguchi
  • Akira Obana
Original Article


Pseudomonas aeruginosa (P. aeruginosa) is a major cause of infectious keratitis, which itself is a major cause of blindness worldwide. We have now evaluated the time-dependent effectiveness of photodynamic antimicrobial chemotherapy (PACT) with the chlorin derivative TONS 504 and a light-emitting diode (LED) on P. aeruginosa in vitro. PACT with TONS 504 (10 mg/L) and irradiation (30 J/m2) by an LED device that delivers light centered on a wavelength of 660 nm was applied to 1 × 106 colony-forming units of P. aeruginosa in liquid medium. The bacteria were then cultured at 37 °C for various times before assay of viability by determination of colony formation on agar plates. The effect of a second irradiation at 3 h after the initial LED exposure was also examined. Bacterial growth was markedly inhibited between 3 and 9 h after PACT with TONS 504, with the maximal effect being apparent at 3 h. Furthermore, a second exposure to LED irradiation at 3 h after the first treatment enhanced the inhibitory effect on bacterial growth. PACT with TONS 504 thus inhibited the growth of P. aeruginosa in a time-dependent manner, and an additional irradiation exposure applied 3 h after the first LED treatment greatly increased the effectiveness of PACT. This antibacterial system thus warrants further evaluation with regard to its potential effectiveness for the treatment of infectious keratitis.


Photodynamic antimicrobial chemotherapy (PACT) Pseudomonas aeruginosa Chlorin derivative TONS 504 Light-emitting diode (LED) Infectious keratitis 



We thank Isao Sakata (Porphyrin Laboratory, Okayama, Japan) for providing information on TONS 504 as well as Akira Ichikawa (CCS, Kyoto, Japan) for building the LED device according to our design.


This work was supported by a Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aid for Scientific Research (C) (no. 15 K10894).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All experimental protocols were approved by the Hiroshima University Animal Experiment Committee, and were in keeping with the basic guidelines of Hiroshima University regarding animal experiments (Approval number: A15–76).


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  • Kentaro Sueoka
    • 1
  • Taiichiro Chikama
    • 1
    Email author
  • Miftahul Akhyar Latief
    • 1
    • 2
  • Ji-Ae Ko
    • 1
  • Yoshiaki Kiuchi
    • 1
  • Takemasa Sakaguchi
    • 3
  • Akira Obana
    • 4
  1. 1.Department of Ophthalmology and Visual Science, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan
  2. 2.Department of OphthalmologyHasanuddin UniversityMakassar CityIndonesia
  3. 3.Department of Virology, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan
  4. 4.Department of OphthalmologySeirei Hamamatsu General HospitalShizuokaJapan

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