Lasers in Medical Science

, Volume 33, Issue 3, pp 549–557 | Cite as

Chondroitin sulfate and glucosamine sulfate associated to photobiomodulation prevents degenerative morphological changes in an experimental model of osteoarthritis in rats

  • Marcella Sanches
  • Lívia Assis
  • Cyntia Criniti
  • Danilo Fernandes
  • Carla Tim
  • Ana Claudia Muniz Renno
Original Article

Abstract

The aim of this study was to compare the effects of combined treatment with chondroitin sulfate and glucosamine sulfate (CS/Gl) and photobiomodulation (PBM) on the degenerative process related to osteoarthritis (OA) in the articular cartilage in rats. Forty male Wistar rats were randomly divided into four groups: OA control group (CG); OA animals submitted to PBM treatment (PBM); OA animals submitted to CS/Gl treatment (CS/Gl); OA submitted to CS/GS associated with PBM treatments (GS/Gl + PBM). The CS/Gl started 48 h after the surgery, and they were performed for 29 consecutive days. Moreover, PBM was performed after the CS/Gl administration on the left joint. Morphological characteristics and immunoexpression of interleukin 10 (IL-10) and 1 beta (IL-1β) and collagen type II (Col II) of the articular cartilage were evaluated. The results showed that all treated groups (CS/Gl and PBM) presented attenuation signs of degenerative process (measured by histopathological analysis) and lower density chondrocytes [PBM (p = 0.0017); CS/Gl (p = 0.0153) and CS/Gl + PBM (p = 0.002)]. Additionally, CS/Gl [associated (p = 0.0089) or not with PBM (p = 0.0059)] showed significative lower values for OARSI grade evaluation. Furthermore, CS/GS + PBM decreased IL-1β protein expression (p = 0.0359) and increased IL-10 (p = 0.028) and Col II imunoexpression (p = 0.0204) compared to CG. This study showed that CS/Gl associated with PBM was effective in modulating inflammatory process and preventing the articular tissue degradation in the knees OA rats.

Keywords

Chondroitin sulfate Glucosamine sulfate Photobiomodulation Articular cartilage Knee osteoarthritis 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2017

Authors and Affiliations

  • Marcella Sanches
    • 1
  • Lívia Assis
    • 1
    • 2
  • Cyntia Criniti
    • 1
  • Danilo Fernandes
    • 1
  • Carla Tim
    • 1
    • 2
  • Ana Claudia Muniz Renno
    • 1
  1. 1.Department of BioscienceFederal University of São PauloSantosBrazil
  2. 2.Department of Biomedical EngineeringBrazil UniversitySão PauloBrazil

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