Lasers in Medical Science

, Volume 33, Issue 2, pp 445–449 | Cite as

Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor

  • Fernanda Gonçalves BassoEmail author
  • Taisa Nogueira Pansani
  • Lais Medeiros Cardoso
  • Mariana Citta
  • Diana Gabriela Soares
  • Débora Salles Scheffel
  • Josimeri Hebling
  • Carlos Alberto de Souza Costa
Brief Report


Reepithelialization and wound closure are the desired outcome for several ulcerative conditions. Such resolution reduces the possibility of wound contamination and maintenance of the injury and improves the reestablishment of tissue morphology and functions. Investigators are seeking adjuvant therapies that can accelerate wound healing and are developing new strategies for clinical applications. This study compared the effects of epidermal growth factor (EGF) application and low-level laser therapy (LLLT) on cultured epithelial cells. Cells were seeded in 24-well plates. After a 24-h incubation, the epithelial cells were either treated with EGF (100 μM in serum-free DMEM for 72 h) or subjected to LLLT (780 nm, 25 mW, 0.5, 1.5, and 3 J/cm2) by three applications every 24 h. Seventy-two hours after cells were treated with EGF or LLLT, cell migration, viability, proliferation, and collagen synthesis were assessed. Cells treated with EGF showed increased cell viability, proliferation, and collagen synthesis compared with those cells that received no treatment. LLLT enhanced cell migration; however, no significant effects of laser irradiation on other cell functions were observed. Comparison of both therapies demonstrated that EGF and LLLT enhanced specific epithelial cell activities related to wound healing.


Epithelial cells Low-level laser therapy Epidermal growth factor Cell proliferation Cell migration 


Compliance with ethical standards


This study was funded by São Paulo State Research Support Foundation – FAPESP (Grant: 2013/05879-0) and the National Council for Scientific and Technological Development – CNPq (Grants: grants: 303599/2014, 307696/2014 and 157779/2015-7).

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval and informed consent

Not applicable.


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Copyright information

© Springer-Verlag London 2017

Authors and Affiliations

  • Fernanda Gonçalves Basso
    • 1
    Email author
  • Taisa Nogueira Pansani
    • 1
  • Lais Medeiros Cardoso
    • 1
  • Mariana Citta
    • 1
  • Diana Gabriela Soares
    • 1
  • Débora Salles Scheffel
    • 2
  • Josimeri Hebling
    • 2
  • Carlos Alberto de Souza Costa
    • 1
  1. 1.Department of Physiology and Pathology, Araraquara School of DensitryUNESPAraraquaraBrazil
  2. 2.Department of Pediatric Dentistry, Araraquara School of DensitryUNESPAraraquaraBrazil

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