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Lasers in Medical Science

, Volume 32, Issue 2, pp 343–349 | Cite as

Effects of different fluences of low-level laser therapy in an experimental model of spinal cord injury in rats

  • Suellen Veronez
  • Lívia AssisEmail author
  • Paula Del Campo
  • Flávia de Oliveira
  • Gláucia de Castro
  • Ana Claudia Muniz Renno
  • Carla Christina Medalha
Original Article

Abstract

The aim of this study was to evaluate the in vivo response of different fluences of low-level laser therapy (LLLT) on the area of the injury, inflammatory markers, and functional recovery using an experimental model of traumatic spinal cord injury (SCI). Thirty two rats were randomly divided into four experimental groups: control group (CG), laser-treated group 500 J/cm2 (L-500), laser-treated group 750 J/cm2 (L-750), and laser-treated group 1000 J/cm2 (L-1000). SCI was performed by an impactor equipment (between the ninth and tenth thoracic vertebrae), with a pressure of 150 kdyn. Afterwards, the injured region was irradiated daily for seven consecutive sessions, using an 808-nm laser, at the respective fluence of each experimental groups. Motor function and tactile sensitivity were performed on days 1 and 7 post-surgery. Animals were euthanized on the eighth day after injury, and the samples were retrieved for histological and immunohistochemistry analyses. Functional evaluation and tactile sensitivity were improved after LLLT, at the higher fluence. Additionally, LLLT, at 750 and 1000 J/cm2, reduces the lesion volume and modulates the inflammatory process with decrease of CD-68 protein expression. These results suggest that LLLT at higher doses was effective in promoting functional recovery and modulating inflammatory process in the spinal cord of rats after SCI.

Keywords

Low-level laser therapy Spinal cord injury Neuronal plasticity Wistar 

Notes

Acknowledgements

We would like to acknowledge the contributions of XXXX funding agency XXXX for the financial support of this research.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Funding

There is no funding source.

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Copyright information

© Springer-Verlag London 2016

Authors and Affiliations

  • Suellen Veronez
    • 1
  • Lívia Assis
    • 1
    Email author
  • Paula Del Campo
    • 1
  • Flávia de Oliveira
    • 1
  • Gláucia de Castro
    • 1
  • Ana Claudia Muniz Renno
    • 1
  • Carla Christina Medalha
    • 1
  1. 1.Department of BioscienceFederal University of São PauloSantosBrazil

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