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Lasers in Medical Science

, Volume 32, Issue 1, pp 189–200 | Cite as

Comparison of the alendronate and irradiation with a light-emitting diode (LED) on murine osteoclastogenesis

  • Hong Moon Sohn
  • Youngjong Ko
  • Mineon Park
  • Bora Kim
  • Jung Eun Park
  • Donghwi Kim
  • Young Lae Moon
  • Wonbong LimEmail author
Original Article

Abstract

Photomodulation therapy (PBMT) using light-emitting diode (LED) has been proposed as an alternative to conventional osteoporosis therapies. Our aim was to determine the effect of irradiation with a light-emitting diode on receptor activator of NF-κB ligand (RANKL)-mediated differentiation of mouse bone marrow macrophages into osteoclasts and compare it to alendronate treatment. The cells were irradiated with LED at 635±10 nm, 9-cm spot size, 5 mW/cm2, and 18 J for 60 min/day in a CO2 incubator. The differentiation of irradiated and untreated RANKL-stimulated bone marrow macrophages into osteoclasts was evaluated by tartrate-resistant acid phosphatase (TRAP) staining and by molecular methods. These included assessing messenger RNA (mRNA) expression of osteoclastic markers such as TRAP, c-Fos, Atp6v0d2, DC-STAMP, NFATc1, cathepsin K, MMP9 and OSCAR; phosphorylation of various MAPKs, including extracellular signal-regulated kinase ERK1/2, P38, and JNK; NF-κB translocation; and resorption pit formation. Results were compared to those obtained with sodium alendronate. Production of reactive oxygen species was measured by a 2’,7’-dihydrodichlorofluorescein diacetate assay. LED irradiation and alendronate inhibited mRNA expression of osteoclast-related genes, such as TRAP, c-Fos, and NFATc1, and reduced the osteoclast activity of RANKL-stimulated bone marrow macrophages. LED irradiation, but not alendronate, also inhibited the production of reactive oxygen species (ROS); phosphorylation of ERK, P38, and IκB; and NF-κB translocation. These findings suggest that LED irradiation downregulates osteoclastogenesis by ROS production; this effect could lead to reduced bone loss and may offer a new therapeutic tool for managing osteoporosis.

Keywords

Osteoporosis LED ROS Osteoclastogenesis 

Notes

Acknowledgments

This study was supported by research funds from Chosun University (2014).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All experimental procedures involving animals were compliant with institutional and governmental requirements and were approved by the Institutional Animal Care and Use Committee (CIACUC2014-A0023) of Chosun University, Gwangju, Korea.

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Copyright information

© Springer-Verlag London 2016

Authors and Affiliations

  • Hong Moon Sohn
    • 1
  • Youngjong Ko
    • 1
  • Mineon Park
    • 1
  • Bora Kim
    • 1
  • Jung Eun Park
    • 2
  • Donghwi Kim
    • 1
  • Young Lae Moon
    • 1
  • Wonbong Lim
    • 1
    • 3
    Email author
  1. 1.Department of Orthopaedic SurgeryChosun University HospitalGwangjuKorea
  2. 2.Department of Biomedical Science, BK21-Plus Research Team for Bioactive ControlChosun UniversityGwangjuKorea
  3. 3.Department of Premedical SciencesCollege of Medicine, Chosun UniversityGwangjuRepublic of Korea

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