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Lasers in Medical Science

, Volume 31, Issue 8, pp 1555–1564 | Cite as

What is the best moment to apply phototherapy when associated to a strength training program? A randomized, double-blinded, placebo-controlled trial

Phototherapy in association to strength training
  • Adriane Aver Vanin
  • Eduardo Foschini Miranda
  • Caroline Santos Monteiro Machado
  • Paulo Roberto Vicente de Paiva
  • Gianna Móes Albuquerque-Pontes
  • Heliodora Leão Casalechi
  • Paulo de Tarso Camillo de Carvalho
  • Ernesto Cesar Pinto Leal-Junior
Original Article

Abstract

The effects of phototherapy (or photobiomodulation therapy) with low-level laser therapy (LLLT) and/or light-emitting diodes (LEDs) on human performance improvement have been widely studied. Few studies have examined its effect on muscular training and no studies have explored the necessary moment of phototherapy irradiations (i.e., before and/or after training sessions). The aim of this study was to determine the optimal moment to apply phototherapy irradiation when used in association with strength training. Forty-eight male volunteers (age between 18 to 35 years old) completed all procedures in this study. Volunteers performed the strength training protocol where either a phototherapy and/or placebo before and/or after each training session was performed using cluster probes with four laser diodes of 905 nm, four LEDs of 875 nm, and four LEDs of 640 nm—manufactured by Multi Radiance Medical™. The training protocol duration was 12 weeks with assessments of peak torque reached in maximum voluntary contraction test (MVC), load in 1-repetition maximum test (1-RM) and thigh circumference (perimetry) at larger cross-sectional area (CSA) at baseline, 4 weeks, 8 weeks, and 12 weeks. Volunteers from group treated with phototherapy before and placebo after training sessions showed significant (p < 0.05) changes in MVC and 1-RM tests for both exercises (leg extension and leg press) when compared to other groups. With an apparent lack of side effects and safety due to no thermal damage to the tissue, we conclude that the application of phototherapy yields enhanced strength gains when it is applied before exercise. The application may have additional beneficial value in post-injury rehabilitation where strength improvements are needed.

Keywords

Photobiomodulation therapy Low-level laser therapy Light-emitting diode Muscle adaptation Muscle fatigue Phototherapy 

Notes

Compliance with ethical standards

All procedures were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in study.

Conflict of interests

Professor Ernesto Cesar Pinto Leal-Junior receives research support from Multi Radiance Medical (Solon, OH, USA), a laser device manufacturer. The remaining authors declare that they have no conflict of interests.

Disclosure of funding received for this work

Adriane Aver Vanin received PhD scholarship from São Paulo Research Foundation (FAPESP) (grant number 2013/19355-3). Caroline Santos Monteiro Machado received undergraduate scholarship from São Paulo Research Foundation (FAPESP) (grant number 2013/25814-0). Professor Ernesto Cesar Pinto Leal-Junior would like to thank São Paulo Research Foundation—FAPESP (grant number 2010/52404-0) and Brazilian Council of Science and Technology Development—CNPq (grant numbers 472062/2013-1 and 307717/2014-3).

References

  1. 1.
    Benson AC, Torode ME, Fiatarone Singh MA (2008) Effects of resistance training on metabolic fitness in children and adolescents: a systematic review. Obes Rev 9:43–66CrossRefPubMedGoogle Scholar
  2. 2.
    Hovanec N, Sawant A, Overend TJ, Petrella RJ, Vandervoort AA (2012) Resistance training and older adults with type 2 diabetes mellitus: strength of the evidence. J Aging Res 2012:284635CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Mota MR, Oliveira RJ, Terra DF, Pardono E, Dutra MT, de Almeida JA, Silva FM (2013) Acute and chronic effects of resistance exercise on blood pressure in elderly women and the possible influence of ACE I/D polymorphism. Int J Gen Med 6:581–587PubMedPubMedCentralGoogle Scholar
  4. 4.
    Nagamatsu LS, Handy TC, Hsu CL, Voss M, Liu-Ambrose T (2012) Resistance training promotes cognitive and functional brain plasticity in seniors with probable mild cognitive impairment. Arch Intern Med 172:666–668CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Rosendahl E, Gustafson Y, Nordin E, Lundin-Olsson L, Nyberg L (2008) A randomized controlled trial of fall prevention by a high-intensity functional exercise program for older people living in residential care facilities. Aging Clin Exp Res 20:67–75CrossRefPubMedGoogle Scholar
  6. 6.
    Murton AJ, Greenhaff PL (2013) Resistance exercise and the mechanisms of muscle mass regulation in humans: acute effects on muscle protein turnover and the gaps in our understanding of chronic resistance exercise training adaptation. Int J Biochem Cell Biol 45:2209–2214CrossRefPubMedGoogle Scholar
  7. 7.
    Hakkinen K, Alen M, Kraemer WJ, Gorostiaga E, Izquierdo M, Rusko H, Mikkola J, Hakkinen A, Valkeinen H, Kaarakainen E, Romu S, Erola V, Ahtiainen J, Paavolainen L (2003) Neuromuscular adaptations during concurrent strength and endurance training versus strength training. Eur J Appl Physiol 89:42–52CrossRefPubMedGoogle Scholar
  8. 8.
    Moritani T, deVries HA (1979) Neural factors versus hypertrophy in the time course of muscle strength gain. Am J Phys Med 58:115–130PubMedGoogle Scholar
  9. 9.
    Seynnes OR, de Boer M, Narici MV (2007) Early skeletal muscle hypertrophy and architectural changes in response to high-intensity resistance training. J Appl Physiol 102:368–373CrossRefPubMedGoogle Scholar
  10. 10.
    Baird MF, Graham SM, Baker JS, Bickerstaff GF (2012) Creatine-kinase- and exercise-related muscle damage implications for muscle performance and recovery. J Nutr Metab 2012:960363CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Peake J, Nosaka K, Suzuki K (2005) Characterization of inflammatory responses to eccentric exercise in humans. Exerc Immunol Rev 11:64–85PubMedGoogle Scholar
  12. 12.
    Yamin C, Duarte JA, Oliveira JM, Amir O, Sagiv M, Eynon N, Sagiv M, Amir RE (2008) IL6 (-174) and TNFA (-308) promoter polymorphisms are associated with systemic creatine kinase response to eccentric exercise. Eur J Appl Physiol 104:579–586CrossRefPubMedGoogle Scholar
  13. 13.
    American College of Sports M (2009) American College of Sports Medicine position stand. Progression models in resistance training for healthy adults. Med Sci Sports Exerc 41:687–708CrossRefGoogle Scholar
  14. 14.
    Campos GE, Luecke TJ, Wendeln HK, Toma K, Hagerman FC, Murray TF, Ragg KE, Ratamess NA, Kraemer WJ, Staron RS (2002) Muscular adaptations in response to three different resistance-training regimens: specificity of repetition maximum training zones. Eur J Appl Physiol 88:50–60CrossRefPubMedGoogle Scholar
  15. 15.
    McBride JM, Blaak JB, Triplett-McBride T (2003) Effect of resistance exercise volume and complexity on EMG, strength, and regional body composition. Eur J Appl Physiol 90:626–632CrossRefPubMedGoogle Scholar
  16. 16.
    Chung H, Dai T, Sharma SK, Huang YY, Carroll JD, Hamblin MR (2012) The nuts and bolts of low-level laser (light) therapy. Ann Biomed Eng 40:516–533CrossRefPubMedGoogle Scholar
  17. 17.
    Huang YY, Chen AC, Carroll JD, Hamblin MR (2009) Biphasic dose response in low level light therapy. Dose Response 7:358–383CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Antonialli FC, de Marchi T, Tomazoni SS, Vanin AA, dos Santos Grandinetti V, de Paiva PR, Pinto HD, Miranda EF, de Tarso C, de Carvalho P, Leal-Junior EC (2014) Phototherapy in skeletal muscle performance and recovery after exercise: effect of combination of super-pulsed laser and light-emitting diodes. Lasers Med Sci 29:1967–1976CrossRefPubMedGoogle Scholar
  19. 19.
    Ferraresi C, de Brito OT, de Oliveira ZL, de Menezes Reiff RB, Baldissera V, de Andrade Perez SE, Matheucci Junior E, Parizotto NA (2011) Effects of low level laser therapy (808 nm) on physical strength training in humans. Lasers Med Sci 26:349–358CrossRefPubMedGoogle Scholar
  20. 20.
    Leal Junior EC, Lopes-Martins RA, Dalan F, Ferrari M, Sbabo FM, Generosi RA, Baroni BM, Penna SC, Iversen VV, Bjordal JM (2008) Effect of 655-nm low-level laser therapy on exercise-induced skeletal muscle fatigue in humans. Photomed Laser Surg 26:419–424CrossRefPubMedGoogle Scholar
  21. 21.
    Leal Junior EC, Lopes-Martins RA, Vanin AA, Baroni BM, Grosselli D, De Marchi T, Iversen VV, Bjordal JM (2009) Effect of 830 nm low-level laser therapy in exercise-induced skeletal muscle fatigue in humans. Lasers Med Sci 24:425–431CrossRefPubMedGoogle Scholar
  22. 22.
    Paolillo FR, Corazza AV, Borghi-Silva A, Parizotto NA, Kurachi C, Bagnato VS (2013) Infrared LED irradiation applied during high-intensity treadmill training improves maximal exercise tolerance in postmenopausal women: a 6-month longitudinal study. Lasers Med Sci 28:415–422CrossRefPubMedGoogle Scholar
  23. 23.
    Toma RL, Tucci HT, Antunes HK, Pedroni CR, de Oliveira AS, Buck I, Ferreira PD, Vassao PG, Renno AC (2013) Effect of 808 nm low-level laser therapy in exercise-induced skeletal muscle fatigue in elderly women. Lasers Med Sci 28:1375–1382CrossRefPubMedGoogle Scholar
  24. 24.
    Vieira WH, Ferraresi C, Perez SE, Baldissera V, Parizotto NA (2012) Effects of low-level laser therapy (808 nm) on isokinetic muscle performance of young women submitted to endurance training: a randomized controlled clinical trial. Lasers Med Sci 27:497–504CrossRefPubMedGoogle Scholar
  25. 25.
    Borsa PA, Larkin KA, True JM (2013) Does phototherapy enhance skeletal muscle contractile function and postexercise recovery? A systematic review. J Athl Train 48:57–67PubMedPubMedCentralGoogle Scholar
  26. 26.
    Leal-Junior EC, Vanin AA, Miranda EF, de Carvalho PT, Dal Corso S, Bjordal JM (2015) Effect of phototherapy (low-level laser therapy and light-emitting diode therapy) on exercise performance and markers of exercise recovery: a systematic review with meta-analysis. Lasers Med Sci 30:925–939CrossRefPubMedGoogle Scholar
  27. 27.
    Folland JP, Williams AG (2007) The adaptations to strength training: morphological and neurological contributions to increased strength. Sports Med 37:145–168CrossRefPubMedGoogle Scholar
  28. 28.
    Kadi F, Charifi N, Denis C, Lexell J, Andersen JL, Schjerling P, Olsen S, Kjaer M (2005) The behaviour of satellite cells in response to exercise: what have we learned from human studies? Pflugers Arch 451:319–327CrossRefPubMedGoogle Scholar
  29. 29.
    Leal-Junior EC (2015) Photobiomodulation therapy in skeletal muscle: from exercise performance to muscular dystrophies. Photomed Laser Surg 33:53–54CrossRefPubMedPubMedCentralGoogle Scholar
  30. 30.
    Ziemann E, Zembron-Lacny A, Kasperska A, Antosiewicz J, Grzywacz T, Garsztka T, Laskowski R (2013) Exercise training-induced changes in inflammatory mediators and heat shock proteins in young tennis players. J Sports Sci Med 12:282–289PubMedPubMedCentralGoogle Scholar
  31. 31.
    Mackey AL (2013) Does an NSAID a day keep satellite cells at bay? J Appl Physiol (1985) 115:900–908CrossRefGoogle Scholar
  32. 32.
    Takagi R, Fujita N, Arakawa T, Kawada S, Ishii N, Miki A (2011) Influence of icing on muscle regeneration after crush injury to skeletal muscles in rats. J Appl Physiol 110:382–388CrossRefPubMedGoogle Scholar
  33. 33.
    Grandinetti Vdos S, Miranda EF, Johnson DS, de Paiva PR, Tomazoni SS, Vanin AA, Albuquerque-Pontes GM, Frigo L, Marcos RL, de Carvalho PT, Leal-Junior EC (2015) The thermal impact of phototherapy with concurrent super-pulsed lasers and red and infrared LEDs on human skin. Lasers Med Sci 30:1575–1581CrossRefPubMedGoogle Scholar
  34. 34.
    Irving BA, Rutkowski J, Brock DW, Davis CK, Barrett EJ, Gaesser GA, Weltman A (2006) Comparison of Borg- and OMNI-RPE as markers of the blood lactate response to exercise. Med Sci Sports Exerc 38:1348–1352CrossRefPubMedGoogle Scholar
  35. 35.
    Abe T, Kojima K, Kearns CF, Yohena H, Fukuda J (2003) Whole body muscle hypertrophy from resistance training: distribution and total mass. Br J Sports Med 37:543–545CrossRefPubMedPubMedCentralGoogle Scholar
  36. 36.
    Ahtiainen JP, Pakarinen A, Alen M, Kraemer WJ, Hakkinen K (2003) Muscle hypertrophy, hormonal adaptations and strength development during strength training in strength-trained and untrained men. Eur J Appl Physiol 89:555–563CrossRefPubMedGoogle Scholar
  37. 37.
    Wernbom M, Augustsson J, Thomee R (2007) The influence of frequency, intensity, volume and mode of strength training on whole muscle cross-sectional area in humans. Sports Med 37:225–264CrossRefPubMedGoogle Scholar
  38. 38.
    Baroni BM, Rodrigues R, Franke RA, Geremia JM, Rassier DE, Vaz MA (2013) Time course of neuromuscular adaptations to knee extensor eccentric training. Int J Sports Med 34:904–911CrossRefPubMedGoogle Scholar
  39. 39.
    Bellamy LM, Joanisse S, Grubb A, Mitchell CJ, McKay BR, Phillips SM, Baker S, Parise G (2014) The acute satellite cell response and skeletal muscle hypertrophy following resistance training. PLoS One 9:e109739CrossRefPubMedPubMedCentralGoogle Scholar
  40. 40.
    Shepstone TN, Tang JE, Dallaire S, Schuenke MD, Staron RS, Phillips SM (2005) Short-term high- vs. low-velocity isokinetic lengthening training results in greater hypertrophy of the elbow flexors in young men. J Appl Physiol 98:1768–1776CrossRefPubMedGoogle Scholar
  41. 41.
    Baroni BM, Rodrigues R, Freire BB, Franke Rde A, Geremia JM, Vaz MA (2015) Effect of low-level laser therapy on muscle adaptation to knee extensor eccentric training. Eur J Appl Physiol 115:639–647CrossRefPubMedGoogle Scholar
  42. 42.
    Albuquerque-Pontes GM, Vieira Rde P, Tomazoni SS, Caires CO, Nemeth V, Vanin AA, Santos LA, Pinto HD, Marcos RL, Bjordal JM, de Carvalho PT, Leal-Junior EC (2015) Effect of pre-irradiation with different doses, wavelengths, and application intervals of low-level laser therapy on cytochrome c oxidase activity in intact skeletal muscle of rats. Lasers Med Sci 30:59–66CrossRefPubMedGoogle Scholar
  43. 43.
    Hayworth CR, Rojas JC, Padilla E, Holmes GM, Sheridan EC, Gonzalez-Lima F (2010) In vivo low-level light therapy increases cytochrome oxidase in skeletal muscle. Photochem Photobiol 86:673–680CrossRefPubMedGoogle Scholar
  44. 44.
    Miranda EF, Vanin AA, Tomazoni SS, Grandinetti Vdos S, de Paiva PR, Machado Cdos S, Monteiro KK, Casalechi HL, de Tarso P, de Carvalho C, Leal-Junior EC (2016) Using pre-exercise photobiomodulation therapy combining super-pulsed lasers and light-emitting diodes to improve performance in progressive cardiopulmonary exercise tests. J Athl Train 51:129–135CrossRefPubMedGoogle Scholar
  45. 45.
    Pinto HD, Vanin AA, Miranda EF, Tomazoni SS, Johnson DS, Albuquerque-Pontes GM, Aleixo Junior IO, Grandinetti VD, Casalechi HL, de Carvalho PT, Leal-Junior EC (2016) Photobiomodulation therapy (PBMT) improves performance and accelerates recovery of high-level Rugby players in field test: a randomized, crossover, double-blind, placebo-controlled clinical study. J Strength Cond Res. [Epub ahead of print]Google Scholar
  46. 46.
    Kwon HJ, Ha YC, Park HM (2015) The reference value of skeletal muscle mass index for defining the sarcopenia of women in Korea. J Bone Metab 22:71–75CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag London 2016

Authors and Affiliations

  • Adriane Aver Vanin
    • 1
    • 2
  • Eduardo Foschini Miranda
    • 1
    • 3
  • Caroline Santos Monteiro Machado
    • 1
  • Paulo Roberto Vicente de Paiva
    • 1
    • 3
  • Gianna Móes Albuquerque-Pontes
    • 1
    • 3
  • Heliodora Leão Casalechi
    • 1
  • Paulo de Tarso Camillo de Carvalho
    • 1
    • 2
    • 3
  • Ernesto Cesar Pinto Leal-Junior
    • 1
    • 2
    • 3
    • 4
  1. 1.Laboratory of Phototherapy in Sports and ExerciseUniversidade Nove de Julho (UNINOVE)São PauloBrazil
  2. 2.Postgraduate Program in Rehabilitation SciencesUniversidade Nove de Julho (UNINOVE)São PauloBrazil
  3. 3.Postgraduate Program in Biophotonics Applied to Health SciencesUniversidade Nove de Julho (UNINOVE)São PauloBrazil
  4. 4.Laboratory of Phototherapy in Sports and ExerciseSão PauloBrazil

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