Lasers in Medical Science

, Volume 31, Issue 7, pp 1363–1370

Laser phototherapy improves early stage of cutaneous wound healing of rats under hyperlipidic diet

  • Virgínia Dias Uzêda-e-Silva
  • Tania Tavares Rodriguez
  • Isadora Almeida Rios Rocha
  • Flávia Calo Aquino Xavier
  • Jean Nunes dos Santos
  • Patrícia Ramos Cury
  • Luciana Maria Pedreira Ramalho
Original Article


The aim of this study was to evaluate the influence of laser photobiomodulation in cutaneous healing of rats under a hyperlipidic diet. Forty-eight Wistar Albinus rats, weaned, received standard diet (SD) or hyperlipidic diet (HD) for 20 weeks. The groups were divided into SD rats and HD rats, SD-irradiated rats (LSD), and HD-irradiated rats (LHD). Standard cutaneous wound (1 cm2) was created on the dorsum of each rat. The irradiation started immediately after surgery and every 48 h for 7 or 14 days (λ660 nm, 40 mW, 6 J/cm2, ϕ 0,04 cm2, CW), when they were killed under deep anesthesia. The specimens were removed, routinely processed, stained with hematoxylin/eosin (H/E), and evaluated by light microscopy. Rats fed with hyperlipidic diet had greater intensity in the inflammatory process and prolonged hyperemia. At day 7, the intensity of inflammation was reduced in LSD and LHD groups when compared to their control groups, SD (p = 0.002) and HD (p = 0.02). There was an increase in fibroblast proliferation and collagen deposition, especially in the LHD group. At day 14, the HD group presented more intensive hyperemia than the SD group. It can be concluded that the hyperlipidic diet modified the inflammation pattern in wound healing and that laser light has a positive biomodulative effect on the healing process only in early stages.


Wound healing Photobiomodulation Laser phototherapy Hyperlipidic diet 


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Copyright information

© Springer-Verlag London 2016

Authors and Affiliations

  • Virgínia Dias Uzêda-e-Silva
    • 1
  • Tania Tavares Rodriguez
    • 2
  • Isadora Almeida Rios Rocha
    • 1
  • Flávia Calo Aquino Xavier
    • 1
  • Jean Nunes dos Santos
    • 1
  • Patrícia Ramos Cury
    • 1
  • Luciana Maria Pedreira Ramalho
    • 1
  1. 1.School of DentistryFederal University of BahiaSalvadorBrazil
  2. 2.Department of Physiology, Health Sciences CenterFederal University of BahiaSalvadorBrazil

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