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Lasers in Medical Science

, Volume 31, Issue 5, pp 841–848 | Cite as

Cell viability, reactive oxygen species, apoptosis, and necrosis in myoblast cultures exposed to low-level infrared laser

  • Larissa Alexsandra da Silva Neto Trajano
  • Camila Luna da Silva
  • Simone Nunes de Carvalho
  • Erika Cortez
  • André Luiz Mencalha
  • Adenilson de Souza da FonsecaEmail author
  • Ana Carolina Stumbo
Original Article

Abstract

Low-level infrared laser is considered safe and effective for treatment of muscle injuries. However, the mechanism involved on beneficial effects of laser therapy are not understood. The aim was to evaluate cell viability, reactive oxygen species, apoptosis, and necrosis in myoblast cultures exposed to low-level infrared laser at therapeutic fluences. C2C12 myoblast cultures at different (2 and 10 %) fetal bovine serum (FBS) concentrations were exposed to low-level infrared laser (808 nm, 100 mW) at different fluences (10, 35, and 70 J/cm2) and evaluated after 24, 48, and 72 h. Cell viability was evaluated by WST-1 assay; reactive oxygen species (ROS), apoptosis, and necrosis were evaluated by flow cytometry. Cell viability was decreased atthe lowest FBS concentration. Laser exposure increased the cell viability in myoblast cultures at 2 % FBS after 48 and 72 h, but no significant increase in ROS was observed. Apoptosis was decreased at the higher fluence and necrosis was increased at lower fluence in myoblast cultures after 24 h of laser exposure at 2 % FBS. No laser-induced alterations were obtained at 10 % FBS. Results show that level of reactive oxygen species is not altered, at least to those evaluated in this study, but low-level infrared laser exposure affects cell viability, apoptosis, and necrosis in myoblast cultures depending on laser fluence and physiologic conditions of cells.

Keywords

Apoptosis Cell viability Reactive oxygen species Low-level laser therapy Myoblast Necrosis 

Notes

Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).

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Copyright information

© Springer-Verlag London 2016

Authors and Affiliations

  • Larissa Alexsandra da Silva Neto Trajano
    • 1
    • 2
  • Camila Luna da Silva
    • 1
  • Simone Nunes de Carvalho
    • 1
  • Erika Cortez
    • 1
  • André Luiz Mencalha
    • 2
  • Adenilson de Souza da Fonseca
    • 2
    • 3
    • 4
    Email author
  • Ana Carolina Stumbo
    • 1
  1. 1.Laboratório de Pesquisa em Células-Tronco, Departamento de Histologia e Embriologia, Instituto de Biologia Roberto Alcantara GomesUniversidade do Estado do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara GomesUniversidade do Estado do Rio de JaneiroRio de JaneiroBrazil
  3. 3.Departamento de Ciências Fisiológicas, Instituto BiomédicoUniversidade Federal do Estado do Rio de JaneiroRio de JaneiroBrazil
  4. 4.Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara GomesUniversidade do Estado do Rio de JaneiroRio de JaneiroBrazil

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