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Lasers in Medical Science

, Volume 31, Issue 5, pp 833–840 | Cite as

The effect of low-level laser therapy on oxidative stress and functional fitness in aged rats subjected to swimming: an aerobic exercise

  • Simone A. Guaraldo
  • Andrey Jorge Serra
  • Eliane Martins Amadio
  • Ednei Luis Antônio
  • Flávio Silva
  • Leslie Andrews Portes
  • Paulo José Ferreira Tucci
  • Ernesto Cesar Pinto Leal-Junior
  • Paulo de Tarso Camillo de CarvalhoEmail author
Original Article

Abstract

The aim of the present study was to determine whether low-level laser therapy (LLLT) in conjunction with aerobic training interferes with oxidative stress, thereby influencing the performance of old rats participating in swimming. Thirty Wistar rats (Norvegicus albinus) (24 aged and six young) were tested. The older animals were randomly divided into aged-control, aged-exercise, aged-LLLT, aged-LLLT/exercise, and young-control. Aerobic capacity (VO2max0.75) was analyzed before and after the training period. The exercise groups were trained for 6 weeks, and the LLLT was applied at 808 nm and 4 J energy. The rats were euthanized, and muscle tissue was collected to analyze the index of lipid peroxidation thiobarbituric acid reactive substances (TBARS), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) activities. VO2 0.75max values in the aged-LLLT/exercise group were significantly higher from those in the baseline older group (p <0.01) and the LLLT and exercise group (p <0.05). The results indicate that the activities of CAT, SOD, and GPx were higher and statistically significant (p <0.05) in the LLLT/exercise group than those in the LLLT and exercise groups. Young animals presented lesser and statistically significant activities of antioxidant enzymes compared to the aged group. The LLLT/exercise group and the LLLT and exercise group could also mitigate the concentration of TBARS (p > 0.05). Laser therapy in conjunction with aerobic training may reduce oxidative stress, as well as increase VO2 0.75max, indicating that an aerobic exercise such as swimming increases speed and improves performance in aged animals treated with LLLT.

Keywords

Low-level laser Aerobic exercise Oxidative stress Aged 

Notes

Acknowledgments

Professor Paulo de Tarso Camillo de Carvalho thanks the Brazilian Research Council-CNPq for the Research Productivity Scholarship (Process no.307665/2012-7).

Compliance with ethical standards

Conflict of interest

No competing financial interests exist. Professor Ernesto Cesar Pinto Leal-Junior receives research support from Multi Radiance Medical (Solon, OH-USA), a laser device manufacturer. Multi Radiance Medical had no role in the planning of this study, and the laser device used was not theirs. They had no influence on study design, data collection and analysis, decision to publish, or preparation of the manuscript. The remaining authors declare that they have no conflict of interests.

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Copyright information

© Springer-Verlag London 2016

Authors and Affiliations

  • Simone A. Guaraldo
    • 1
  • Andrey Jorge Serra
    • 2
  • Eliane Martins Amadio
    • 2
  • Ednei Luis Antônio
    • 3
  • Flávio Silva
    • 3
  • Leslie Andrews Portes
    • 3
  • Paulo José Ferreira Tucci
    • 3
  • Ernesto Cesar Pinto Leal-Junior
    • 1
    • 2
  • Paulo de Tarso Camillo de Carvalho
    • 1
    • 2
    Email author
  1. 1.Postgraduate Program in Rehabilitation SciencesUniversidade Nove de Julho (UNINOVE)São PauloBrazil
  2. 2.Postgraduate Program in Biophotonics Applied Health SciencesUniversidade Nove de Julho (UNINOVE)São PauloBrazil
  3. 3.Department of CardiologyFederal University of São Paulo (UNIFESP)São PauloBrazil

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