Lasers in Medical Science

, Volume 30, Issue 3, pp 1061–1068 | Cite as

Effect of low-level laser therapy on bone repair: a randomized controlled experimental study

  • Valéria Regina Gonzalez Sella
  • Fernando Russo Costa do Bomfim
  • Paula Carolina Dias Machado
  • Maria José Misael da Silva Morsoleto
  • Milton Chohfi
  • Helio Plapler
Original Article


The aim of this study was to investigate the effect of low-level laser therapy (LLLT) on bone repair in femoral fractures. Sixty adult Wistar rats were randomly assigned into one of two groups: group A (ostectomy + LLLT) or group B (ostectomy + sham laser). An experimental model of complete bone fracture was surgically created by removing a 2-mm fragment from the middle third of the femoral shaft. Data were analyzed on days 8, 13, and 18 after the fracture (subgroups 1, 2, and 3). Samples were assessed for changes in inflammatory infiltration; trabecular bone matrix, periosteal, and new bone formations; and changes in the expression of particular osteogenic-related proteins (osteocalcin, osteopontin, and osteonectin). Microscopic analysis revealed a significant decrease in inflammatory infiltration, intense trabecular bone matrix and periosteal formation, and an increase in newly formed bone after laser irradiation. We also found an increase in the expression of bone matrix proteins with LLLT, with a significant difference measured for osteocalcin in the LLLT group at day 8 (p = 0.007). We show that LLLT plays an important role in augmenting bone tissue formation, which is relevant to fracture healing. LLLT may therefore be indicated as an adjunct therapeutic tool in clinical practice for the treatment or recovery of nonunion injuries.


Bone remodeling Femoral fracture Low-level laser therapy 


Ethical approval

Animal manipulation was performed in accordance with the animal testing guide (in agreement with the Brazilian Legislation no. 11.794/2008 for Procedures for the Scientific Use of Animals). This randomized controlled experimental study was previously approved by the Research Ethics Committee of Federal University of São Paulo under no. 1101/09.

Conflict of interest

All authors have no conflicts of interest.


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Copyright information

© Springer-Verlag London 2015

Authors and Affiliations

  • Valéria Regina Gonzalez Sella
    • 1
    • 3
  • Fernando Russo Costa do Bomfim
    • 1
  • Paula Carolina Dias Machado
    • 1
  • Maria José Misael da Silva Morsoleto
    • 1
  • Milton Chohfi
    • 2
  • Helio Plapler
    • 1
  1. 1.Department of Surgery, Division of Operative Technique and Experimental SurgeryUniversidade Federal de São Paulo [Federal University of São Paulo] – UNIFESPSão PauloBrazil
  2. 2.Department of OrthopedicsUniversidade Federal de São Paulo [Federal University of São Paulo] – UNIFESPSão PauloBrazil
  3. 3.São PauloBrazil

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