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Lasers in Medical Science

, Volume 30, Issue 7, pp 1855–1866 | Cite as

Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis

  • Mayumi Ogita
  • Sachio TsuchidaEmail author
  • Akira AokiEmail author
  • Mamoru Satoh
  • Sayaka Kado
  • Masanori Sawabe
  • Hiromi Nanbara
  • Hiroaki Kobayashi
  • Yasuo Takeuchi
  • Koji Mizutani
  • Yoshiyuki Sasaki
  • Fumio Nomura
  • Yuichi Izumi
Original Article

Abstract

Erbium-doped yttrium aluminum garnet (Er:YAG) laser treatment has demonstrated favorable wound healing effect after periodontal therapy. One of the reasons may be the positive biological effect of the low-level laser on the irradiated tissues, although the mechanism remains unclear. The aim of this study was to investigate the effect of low-level Er:YAG laser irradiation on cell proliferation and laser-induced differential expression of proteins in human gingival fibroblasts (HGFs) by proteomic analysis. In the first experiment, HGFs were exposed to low-level Er:YAG laser irradiation and the laser-induced cell proliferation and damage were evaluated on day 3. In the second experiment, proteomic analysis was performed on day 1 after irradiation. The peptides prepared from HGFs were analyzed by a hybrid ion trap-Fourier transform mass spectrometer, Mascot search engine, and UniProtKB database. A significant increase in cell proliferation without cell damage after irradiation was observed. Among the total identified 377 proteins, 59 proteins, including galectin-7, which was associated with the process of wound healing, were upregulated and 15 proteins were downregulated in laser-treated HGFs. In the third experiment, the increase in messenger RNA (mRNA) and protein expression of galectin-7 in the irradiated HGFs was validated by various analytical techniques. In addition, the effect of recombinant human galectin-7 on the modulation of HGFs proliferation was confirmed. The results indicate that low-level Er:YAG laser irradiation can promote HGF proliferation and induce a significant change in protein expression and the upregulation of galectin-7 expression may partly contribute to the increase in cell proliferation.

Keywords

Er:YAG laser Galectin-7 Gingival fibroblasts Low-level laser therapy Proteomics 

Notes

Acknowledgments

This study was partially supported by the grant of the Global Center of Excellence Program of the International Research Center for Molecular Science in Tooth and Bone Diseases at TMDU funded by the Ministry of Education, Culture, Sports, Science and Technology of Japan and the grant of Grants-in-Aid for Scientific Research (C) (no. 22392308 and no. 23463212 for A.A.) from the Japan Society for the Promotion of Science. We would like to acknowledge Dr. Kengo Iwasaki and Dr. Yoichi Taniguchi at TMDU for their kind advices and supports.

Supplementary material

10103_2014_1691_MOESM1_ESM.pdf (186 kb)
Table 1 LC-MS/MS-identified 377 proteins in HGFs with and/or without low-level Er:YAG laser irradiation (PDF 185 kb)

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Copyright information

© Springer-Verlag London 2014

Authors and Affiliations

  • Mayumi Ogita
    • 1
    • 5
  • Sachio Tsuchida
    • 2
    Email author
  • Akira Aoki
    • 1
    Email author
  • Mamoru Satoh
    • 2
  • Sayaka Kado
    • 3
  • Masanori Sawabe
    • 1
  • Hiromi Nanbara
    • 1
  • Hiroaki Kobayashi
    • 1
  • Yasuo Takeuchi
    • 1
  • Koji Mizutani
    • 1
  • Yoshiyuki Sasaki
    • 4
  • Fumio Nomura
    • 2
  • Yuichi Izumi
    • 1
    • 5
  1. 1.Department of Periodontology, Graduate School of Medical and Dental SciencesTokyo Medical and Dental University (TMDU)Bunkyo-kuJapan
  2. 2.Department of Molecular Diagnosis (F8), Graduate School of MedicineChiba UniversityChibaJapan
  3. 3.Chemical Analysis CenterChiba UniversityChibaJapan
  4. 4.Maxillofacial Orthognathics, Department of Maxillofacial and Neck Reconstruction, Graduate School of Medical and Dental SciencesTMDUBunkyo-kuJapan
  5. 5.Global Center of Excellence Program of the International Research Center for Molecular Science in Tooth and Bone Diseases, Graduate School of Medical and Dental SciencesTMDUBunkyo-kuJapan

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