Lasers in Medical Science

, Volume 30, Issue 1, pp 383–387 | Cite as

Antimicrobial action from a novel porphyrin derivative in photodynamic antimicrobial chemotherapy in vitro

  • Miftahul Akhyar Latief
  • Taiichiro ChikamaEmail author
  • Momoko Shibasaki
  • Takaaki Sasaki
  • Ji-Ae Ko
  • Yoshiaki Kiuchi
  • Takemasa Sakaguchi
  • Akira Obana
Original Article


Efforts to identify improved treatments for corneal infection include the development of photodynamic antimicrobial chemotherapy (PACT). We evaluated the antimicrobial effect of PACT with a novel porphyrin derivative, TONS 504, and a novel light system on methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). Bacteria were irradiated with a light-emitting diode (LED) at energies of 10, 20, or 30 J/cm2 in the presence of various concentrations of TONS 504. Bacterial viability was assessed at 30 min and 24 h after irradiation by determination of colony formation on agar plates. PACT inhibited the growth of both MSSA and MRSA as early as 30 min after light exposure. Complete inhibition of bacterial growth was apparent at 24 h after irradiation at a TONS 504 concentration of 1 mg/L and LED energies of ≥10 J/cm2 or a TONS 504 concentration of 0.5 mg/L and LED energies of ≥20 J/cm2 for MSSA, and at a TONS 504 concentration of 10 mg/L and LED energies of ≥10 J/cm2 or of a TONS 504 concentration of 1 mg/L and LED energies of ≥20 J/cm2 for MRSA. Bacterial growth was unaffected by TONS 504 in the absence of irradiation or by irradiation in the absence of TONS 504. Our results thus demonstrate the antimicrobial efficacy of PACT with TONS 504 and a LED against both MSSA and MRSA in vitro, and they therefore provide a basis for further investigation of this system as a potential treatment for corneal infection.





We thank Isao Sakata (Porphyrin Laboratory, Okayama, Japan) for providing information of TONS 504 as well as Akira Ichikawa (CCS Inc., Kyoto, Japan) for creating the LED device according to our experimental design.


This work is supported by Adaptable and Seamless Technology transfer Program through target-driven R&D (A-STEP) of the Japan Science and Technology Agency (JST).

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag London 2014

Authors and Affiliations

  • Miftahul Akhyar Latief
    • 1
    • 2
    • 3
  • Taiichiro Chikama
    • 1
    Email author
  • Momoko Shibasaki
    • 1
  • Takaaki Sasaki
    • 1
  • Ji-Ae Ko
    • 1
  • Yoshiaki Kiuchi
    • 1
  • Takemasa Sakaguchi
    • 4
  • Akira Obana
    • 5
  1. 1.Department of Ophthalmology and Visual Science, Graduate School of Biomedical SciencesHiroshima UniversityMinami-KuJapan
  2. 2.Medical FacultyMuhammadiyah UniversityMakassar CityIndonesia
  3. 3.Department of OphthalmologyHasanuddin UniversityMakassar CityIndonesia
  4. 4.Department of Virology, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan
  5. 5.Department of OphthalmologySeirei Hamamatsu General HospitalShizuokaJapan

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