In vitro assessment of effects of hyperglycemia on the optical properties of blood during coagulation using optical coherence tomography
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No published reports have demonstrated the capability of the optical coherence tomography technique for quantifying the optical coherence tomography signal slope, 1/e light penetration depth, and attenuation coefficient of hyperglycemic blood by an in vitro assessment. The purpose of this study was to investigate the effects of hyperglycemia on optical properties during in vitro blood coagulation by optical coherence tomography. Normal whole blood acted as the control group. After 1-h coagulation, the average optical coherence tomography signal slope decreased approximately 23.3 and 16.7 %, and the 1/e light penetration depths increased approximately 21.5 and 19.2 % for the control and hyperglycemic groups, respectively. It could be seen from the 1/e light penetration depth evolution curves that the blood coagulation time was about (425 ± 19) s for normal whole blood and (367 ± 15) s for the hyperglycemic blood. The coagulation time decreased 13.6 % for the hyperglycemic blood compared with that for normal whole blood. There was statistically significant difference in blood coagulation time between the hyperglycemic and normal whole blood (p < 0.05). The results suggested that hyperglycemia has a procoagulant effect. Our experiment was the first reported study of monitoring hyperglycemic blood coagulation using OCT. We conclude that OCT is potential technique to quantify and follow the liquid-gel transition of hyperglycemic blood coagulation.
KeywordsBlood Coagulation Hyperglycemia Optical coherence tomography
This work was supported by the National Natural Science Foundation of China (Grant Nos. 61335011, 61275187, and 81071790), Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20114407110001 and 200805740003), the Science and Technology Innovation Project of the Education Department of Guangdong Province of China, and Key Laboratory of Optoelectronic Science and Technology for Medicine (Fujian Normal University), Ministry of Education, China (Grant No. JYG1202)
- 2.Hoyert DL, Kung H, Smith BL (2005) Deaths: preliminary data for 2003. Natl Vital Stat Rep 53(15):1–48Google Scholar
- 17.Brezinski ME, Tearney GJ, Weissman NJ, Boppart SA, Bouma BE, Hee MR, Weyman AE, Swanson EA, Southern JF, Fujimoto JG (1997) Assessing atherosclerotic plaque morphology: comparison of optical coherence tomography and high frequency intravascular ultrasound. Heart 77(5):397–403PubMedCentralPubMedGoogle Scholar