Lasers in Medical Science

, Volume 30, Issue 4, pp 1251–1257 | Cite as

The effect of low-level laser therapy (810 nm) on root development of immature permanent teeth in dogs

  • Reza Fekrazad
  • Bahman Seraj
  • Sara Ghadimi
  • Parvin Tamiz
  • Pouriya Mottahary
  • Mohammad-Mehdi Dehghan
Original Article


Traumatic injuries and dental caries can be a big challenge to immature teeth. In these cases, the main purpose of treatment is to maintain the pulp vitality. The purpose of this study was to investigate the effect of low-level laser therapy on accelerating the rate of dentinogenesis in pulpotomy of immature permanent teeth (apexogenesis). Three dogs, 4–6 months old, were used in this study. One jaw in each dog was randomly assigned to laser irradiation group. All selected teeth were pulpotomized with mineral trioxide aggregate (MTA) and restored with amalgam. In the laser group, the Ga-Al-As laser (810 nm, 0.3 W, 4 J/cm2, 9 s) was used on buccal and lingual gingiva of each tooth in 48 h intervals for 2 weeks. In order to observe the newly formed dentine, tetracycline was injected on the 1st, 3rd, 7th, and 14th day after the operation. Then, ground sections of teeth were observed under a fluorescence microscope. The data was analyzed with Generalized Estimating Equations (GEE) test. The mean distance between the lines of tetracycline formed on the 1st and 14th day was significantly higher in the laser group (P = 0.005). Within the limitation of this study, irradiation of Ga-Al-As laser (810 nm) can accelerate the rate of dentinogenesis in apexogenesis of immature permanent teeth with MTA in dogs.


Pulpotomy Low-level laser therapy Mineral trioxide aggregate Dog 



This project has been supported by Tehran University of Medical Sciences & health Services grant number 89-04-97-12065.


  1. 1.
    Torabinejad M, Walton RE (2009) Endodontics: principles and practice. Elsevier Science Health Science Division. p 31Google Scholar
  2. 2.
    Witherspoon DE, Small JC, Harris GZ (2006) Mineral trioxide aggregate pulpotomies: a case series outcomes assessment. J Am Dent Assoc 137:610–618CrossRefPubMedGoogle Scholar
  3. 3.
    Patel R, Cohenca N (2006) Maturogenesis of a cariously exposed immature permanent tooth using MTA for direct pulp capping: a case report. Dent Traumatol 22:328–333CrossRefPubMedGoogle Scholar
  4. 4.
    Webber RT (1984) Apexogenesis versus apexification. Dent Clin N Am 28:669–697PubMedGoogle Scholar
  5. 5.
    Andreasen JO, Frances M. Andreasen FM, Andersson L (2007) Textbook and color atlas of traumatic injuries to the teeth. Blackwell Munksgaard. p 86–87.Google Scholar
  6. 6.
    El-Meligy OA, Avery DR (2006) Comparison of mineral trioxide aggregate and calcium hydroxide as pulpotomy agents in young permanent teeth (apexogenesis). Pediatr Dent 28:399–404PubMedGoogle Scholar
  7. 7.
    Horsted-Bindslev P, Vilkinis V, Sidlauskas A (2003) Direct capping of human pulps with a dentin bonding system or with calcium hydroxide cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 96:591–600CrossRefPubMedGoogle Scholar
  8. 8.
    Barrieshi-Nusair KM, Qudeimat MA (2006) A prospective clinical study of mineral trioxide aggregate for partial pulpotomy in cariously exposed permanent teeth. J Endod 32:731–735CrossRefPubMedGoogle Scholar
  9. 9.
    Toomarian L, Fekrazad R, Tadayon N, Ramezani J, Tuner J (2012) Stimulatory effect of low-level laser therapy on root development of rat molars: a preliminary study. Lasers Med Sci 27:537–542CrossRefPubMedGoogle Scholar
  10. 10.
    Linde A, Goldberg M (1993) Dentinogenesis. Crit Rev Oral Biol Med 4:679–728PubMedGoogle Scholar
  11. 11.
    Martens LC (2011) Laser physics and a review of laser applications in dentistry for children. Eur Arch Paediatr Dent 12:61–67CrossRefPubMedGoogle Scholar
  12. 12.
    Utsunomiya T (1998) A histopathological study of the effects of low-power laser irradiation on wound healing of exposed dental pulp tissues in dogs, with special reference to lectins and collagens. J Endod 24:187–193CrossRefPubMedGoogle Scholar
  13. 13.
    Ohbayashi E, Matsushima K, Hosoya S, Abiko Y, Yamazaki M (1999) Stimulatory effect of laser irradiation on calcified nodule formation in human dental pulp fibroblasts. J Endod 25:30–33CrossRefPubMedGoogle Scholar
  14. 14.
    Ferreira AN, Silveira L, Genovese WJ, de Araujo VC, Frigo L, de Mesquita RA et al (2006) Effect of GaAIAs laser on reactional dentinogenesis induction in human teeth. Photomed Laser Surg 24:358–365CrossRefPubMedGoogle Scholar
  15. 15.
    Toomarian L, Fekrazad R, Sharifi D, Baghaei M, Rahimi H, Eslami B (2008) Histopathological evaluation of pulpotomy with Er, Cr:YSGG laser vs formocresol. Lasers Med Sci 23:443–450CrossRefPubMedGoogle Scholar
  16. 16.
    Torabinejad M, Corr R, Buhrley M, Wright K, Shabahang S (2011) An animal model to study regenerative endodontics. J Endod 37:197–202CrossRefPubMedGoogle Scholar
  17. 17.
    Dissanayaka WL, Zhu X, Zhang C, Jin L (2011) Characterization of dental pulp stem cells isolated from canine premolars. J Endod 37:1074–1080CrossRefPubMedGoogle Scholar
  18. 18.
    Sanchez AR, Rogers RS 3rd, Sheridan PJ (2004) Tetracycline and other tetracycline-derivative staining of the teeth and oral cavity. Int J Dermatol 43:709–715CrossRefPubMedGoogle Scholar
  19. 19.
    Bevelander G, Nakahara H (1966) The effect of diverse amounts of tetracycline on fluorescence and coloration of teeth. J Pediatr 68:114–120CrossRefPubMedGoogle Scholar
  20. 20.
    Harcourt JK, Johnson NW, Storey E (1962) In vivo incorporation of tetracycline in the teeth of man. Arch Oral Biol 7:431–437CrossRefPubMedGoogle Scholar
  21. 21.
    Bennett IC, Law DB (1965) Incorporation of tetracycline in developing dog enamel and dentin. J Dent Res 44:788–793CrossRefPubMedGoogle Scholar
  22. 22.
    Tate Y, Yoshiba K, Yoshiba N, Iwaku M, Okiji T, Ohshima H (2006) Odontoblast responses to GaAlAs laser irradiation in rat molars: an experimental study using heat-shock protein-25 immunohistochemistry. Eur J Oral Sci 114:50–57CrossRefPubMedGoogle Scholar
  23. 23.
    Matsui S, Tsujimoto Y, Matsushima K (2007) Stimulatory effects of hydroxyl radical generation by Ga-Al-As laser irradiation on mineralization ability of human dental pulp cells. Biol Pharm Bull 30:27–31CrossRefPubMedGoogle Scholar
  24. 24.
    Sun G, Tuner J (2004) Low-level laser therapy in dentistry. Dent Clin N Am 48:1061–1076CrossRefPubMedGoogle Scholar
  25. 25.
    Godoy BM, Arana-Chavez VE, Nunez SC, Ribeiro MS (2007) Effects of low-power red laser on dentine–pulp interface after cavity preparation. An ultrastructural study. Arch Oral Biol 52:899–903CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag London 2014

Authors and Affiliations

  • Reza Fekrazad
    • 1
  • Bahman Seraj
    • 2
  • Sara Ghadimi
    • 3
  • Parvin Tamiz
  • Pouriya Mottahary
  • Mohammad-Mehdi Dehghan
    • 4
  1. 1.Laser Research Center in Medical SciencesAJA University of Medical SciencesTehranIslamic Republic of Iran
  2. 2.Dental Research Center, Department of Pediatric Dentistry, Faculty of DentistryTehran University of Medical SciencesTehranIslamic Republic of Iran
  3. 3.Laser Research Center of Dentistry, Department of Pediatric Dentistry, Faculty of DentistryTehran University of Medical SciencesTehranIslamic Republic of Iran
  4. 4.Department of Veterinary Surgery and Radiology, Faculty of Veterinary MedicineUniversity of TehranTehranIslamic Republic of Iran

Personalised recommendations