Lasers in Medical Science

, Volume 29, Issue 5, pp 1685–1693 | Cite as

Effects of laser irradiation (670-nm InGaP and 830-nm GaAlAs) on burn of second-degree in rats

  • Gabriela Bortolança Chiarotto
  • Lia Mara Grosso Neves
  • Marcelo Augusto Marreto Esquisatto
  • Maria Esméria Corezola do Amaral
  • Gláucia Maria Tech dos Santos
  • Fernanda Aparecida Sampaio Mendonça
Original Article


This study investigated the effects of 670-nm indium gallium phosphide (InGaP) and 830-nm gallium aluminum arsenide (GaAlAs) laser therapy on second-degree burns induced on the back of Wistar rats. Sixty-three male Wistar rats were anesthetized, and second-degree burns were made on their back. The animals were then divided randomly into three groups: control (C), animals treated with 670-nm InGaP laser (LIn), and animals treated with 830-nm GaAlAs laser (LGa). The wound areas were removed after 2, 6, 10, 14, and 18 days of treatment and submitted to structural and morphometric analysis. The following parameters were studied: total number of granulocytes and fibroblasts, number of newly formed blood vessels, and percentage of birefringent collagen fibers in the repair area. Morphometric analysis showed that different lasers 670-nm InGaP and 830-nm GaAlAs reduced the number of granulocytes and an increase of newly formed vessels in radiated lesions. The 670-nm InGaP laser therapy was more effective in increasing the number of fibroblasts. The different treatments modified the expression of VEGF and TGF-β1, when compared with lesions not irradiated. The different types of light sources showed similar effects, improved the healing of second-degree burns and can help for treating this type of injury. Despite the large number of studies with LLTI application in second-degree burns, there is still divergence about the best irradiation parameters to be used. Further studies are needed for developing a protocol effective in treating this type of injury.


Lasers Burns Western blotting Rats 



We thank the Hermínio Ometto University Center, UNIARARAS and Institutional Program of Scientific Initiation Scholarships/National Council for Technological and Scientific Development (PIBIC/CNPQ) for supporting this study.

Conflicts of interest

No conflicting interests exist.


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Copyright information

© Springer-Verlag London 2014

Authors and Affiliations

  • Gabriela Bortolança Chiarotto
    • 1
  • Lia Mara Grosso Neves
    • 2
  • Marcelo Augusto Marreto Esquisatto
    • 2
  • Maria Esméria Corezola do Amaral
    • 2
  • Gláucia Maria Tech dos Santos
    • 2
  • Fernanda Aparecida Sampaio Mendonça
    • 2
  1. 1.School of BiomedicineHerminio Ometto University Center, UNIARARASArarasBrazil
  2. 2.Postgraduate Program of Biomedical SciencesHerminio Ometto University Center, UNIARARASArarasBrazil

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