Lasers in Medical Science

, Volume 29, Issue 5, pp 1685–1693 | Cite as

Effects of laser irradiation (670-nm InGaP and 830-nm GaAlAs) on burn of second-degree in rats

  • Gabriela Bortolança Chiarotto
  • Lia Mara Grosso Neves
  • Marcelo Augusto Marreto Esquisatto
  • Maria Esméria Corezola do Amaral
  • Gláucia Maria Tech dos Santos
  • Fernanda Aparecida Sampaio Mendonça
Original Article

Abstract

This study investigated the effects of 670-nm indium gallium phosphide (InGaP) and 830-nm gallium aluminum arsenide (GaAlAs) laser therapy on second-degree burns induced on the back of Wistar rats. Sixty-three male Wistar rats were anesthetized, and second-degree burns were made on their back. The animals were then divided randomly into three groups: control (C), animals treated with 670-nm InGaP laser (LIn), and animals treated with 830-nm GaAlAs laser (LGa). The wound areas were removed after 2, 6, 10, 14, and 18 days of treatment and submitted to structural and morphometric analysis. The following parameters were studied: total number of granulocytes and fibroblasts, number of newly formed blood vessels, and percentage of birefringent collagen fibers in the repair area. Morphometric analysis showed that different lasers 670-nm InGaP and 830-nm GaAlAs reduced the number of granulocytes and an increase of newly formed vessels in radiated lesions. The 670-nm InGaP laser therapy was more effective in increasing the number of fibroblasts. The different treatments modified the expression of VEGF and TGF-β1, when compared with lesions not irradiated. The different types of light sources showed similar effects, improved the healing of second-degree burns and can help for treating this type of injury. Despite the large number of studies with LLTI application in second-degree burns, there is still divergence about the best irradiation parameters to be used. Further studies are needed for developing a protocol effective in treating this type of injury.

Keywords

Lasers Burns Western blotting Rats 

Notes

Acknowledgments

We thank the Hermínio Ometto University Center, UNIARARAS and Institutional Program of Scientific Initiation Scholarships/National Council for Technological and Scientific Development (PIBIC/CNPQ) for supporting this study.

Conflicts of interest

No conflicting interests exist.

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Copyright information

© Springer-Verlag London 2014

Authors and Affiliations

  • Gabriela Bortolança Chiarotto
    • 1
  • Lia Mara Grosso Neves
    • 2
  • Marcelo Augusto Marreto Esquisatto
    • 2
  • Maria Esméria Corezola do Amaral
    • 2
  • Gláucia Maria Tech dos Santos
    • 2
  • Fernanda Aparecida Sampaio Mendonça
    • 2
  1. 1.School of BiomedicineHerminio Ometto University Center, UNIARARASArarasBrazil
  2. 2.Postgraduate Program of Biomedical SciencesHerminio Ometto University Center, UNIARARASArarasBrazil

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